糖蛋白的质谱分析策略  

作　　者：蔡耘[1] 代景泉[1] 张养军[1] 卢庄[1] 王京兰[1] 应万涛[1] 钱小红[1] 

机构地区：[1]军事医学科学院放射医学研究所．北京100850

出　　处：《质谱学报》2004年第B10期131-132,共2页Journal of Chinese Mass Spectrometry Society

摘　　要：Glycoprotein is a kind of proteins widely distributed in the bodies of animals and plants. The identification of its characteristic is very difficult due to the complexity of its structure. In this paper, a serious of methods for glycoprotein analysis was established by mass spectrum. The molecular weight, quantity of N-glycans and sialic acids were determined by matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOFMS) directly. The glycosylation sites of glycoprotein were confirmed by its PMF,which produced by the combination digestion of PNGase F and Endoproteinase. The sequence of glycopeptide was detected by liquid chromatography-mass spectrometry (LCMS). The recombined biotechnologic productions including rhEPO,proUK and Spike protein of SARS coronavirus were well characterized by using these methods.

关 键 词：糖蛋白 质谱分析 分子量 糖基化位点 糖链结构 

分 类 号：O629.73[理学—有机化学] O657.63[理学—化学]