基质辅助激光解吸附飞行时间串联质谱法测定rhEPO唾液酸含量及鉴定糖基化位点  

作　　者：卢臣[1] 蔡耘[1] 代景泉[1] 王京兰[1] 张养军[1] 钱小红[1] 

机构地区：[1]军事医学科学院放射医学研究所，北京100850

出　　处：《质谱学报》2004年第B10期139-140,共2页Journal of Chinese Mass Spectrometry Society

摘　　要：Erythropoietin (EPO) is a glycoprotein with three N-glycosylation sites and one O-glycosylation sites. According to the reference, all of the N-glyco-chains were acid complex type. The amount of sialic acid in rhEPO was determined and two N-glycosylation sites on it were confirmed by matrix-assisted laser desorption time of flight-time of flight mass spectrometry (MALDI-TOF-TOFMS) in this paper. The total amount of sialic acid in rhEPO accounts for 12.97%. Two N-glycosylation sites (Asn38,Asn83) were identified successfully.

关 键 词：基质辅助激光解吸附飞行时间串联质谱 唾液酸 含量测定 糖基化位点鉴定 重组促红细胞生成素 糖蛋白 

分 类 号：O629.73[理学—有机化学] Q513.2[理学—化学]