乙型肝炎病毒感染者树突状细胞(DC)亚群的变化及意义  

Characterization and biological significance of peripheral dendritic cell subsets in human hepatitis B infection

作　　者：段学章[1] 李捍卫[1] 王敏[1] 王福生[1] 

机构地区：[1]解放军第302医院生物治疗研究中心,北京100039

出　　处：《中华微生物学和免疫学杂志》2004年第11期892-896,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目 ( 3 0 2 712 3 0 )

摘　　要：目的　分析乙型肝炎病毒 (HBV)感染引起的慢性肝炎和肝炎肝硬变患者体内树突状细胞 (DC)亚群 (包括MDC和PDC)的数量和功能变化。方法　采用流式细胞分析技术检测 12例急性乙型肝炎、4 3例慢性乙型肝炎、15例肝炎肝硬变患者及 2 2例健康人体内MDC和PDC细胞的比例和数量 ;体外培养患者及健康人外周血DC1并检测其表型和刺激混合淋巴细胞反应 (MLR)的能力 ,评价MDC的功能 ;用 1型单纯疱疹病毒 (HSV 1)刺激外周血单个核细胞 (PBMCs)并与PBMCs共培养 ,通过检测PBMCs产生的α 干扰素水平评估PDC的功能。结果　慢性乙型肝炎患者的DC1表面CD80、CD86的表达水平低于健康人 ;健康人外周血MDC的比例为 0 .4 5 %± 0 .14 % ,绝对数为 (11.3± 6 .3)× 10 6个 L ,两者在肝炎肝硬变患者下降 ;而健康人外周血PDC的比例为 0 .36 %± 0 .15 % ,绝对数为 (8.9±4 .2 )× 10 6 个 L ,两者在慢性肝炎和肝炎肝硬变患者均下降 ;慢性肝炎和肝硬变患者PDC分泌的α 干扰素量也低于健康人。结论　乙肝病毒感染慢性化后 ,患者体内两种DC均出现数量和功能异常 。Objective To investigate the quantity and function of circulating dendritic cell (DC) subsets in patients with chronic hepatitis B virus (HBV) infection and to analyze their biological significance. Methods Phenotype molecules of DC1 were determined both in patients with chronic HBV infection and healthy people. We also used PBMCs to investigate PDC function. IFN-α production of HBV-infected patients and normal controls was determined by ELISA method. Results The frequency and cell number of circulating MDC were 0.45%±(0.14%) and (11.3±6.3)×10~6/L separately in healthy people,which decreased in patients with liver cirrhosis (LC) compared with that in normal controls. However, PDC frequency and number were 0.36%±0.15% and (8.9±4.2)×10~6/L,which decreased both in patients with chronic hepatitis B (CHB) and LC. DC1 cultured from CHB patients showed less expression of CD80 and CD86 in comparison with that in normal controls. The ability of PBMCs to secrete IFN-α also significantly decreased in patients. Conclusion The results suggested that patients with chronic HBV infection have a quantitative and qualitative change of DC subsets, which may partly contribute to HBV disease progression in these patients. [

关 键 词：患者 健康人 肝炎肝硬变 人外周血 体内 慢性肝炎 亚群 表达水平 细胞 共培养 

分 类 号：R512.62[医药卫生—内科学] R392[医药卫生—临床医学]