TRAIL及TRAIL受体在慢性乙型肝炎患者外周血淋巴细胞的表达  

The mRNA expression of TRAIL and TRAIL-R in the PBL of patients with chronic hepatitis B

作　　者：刘征波[1] 范学工[1] 胡国龄[1] 

机构地区：[1]中南大学湘雅医院传染科,长沙410008

出　　处：《中华微生物学和免疫学杂志》2004年第11期897-900,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的　探讨TRAIL(TNF relatedapoptosis inducingligand)及TRAIL受体 (TRAIL R)mRNA在慢性乙型肝炎患者外周血淋巴细胞 (peripheralbloodlymphocytes,PBL)中的表达。方法　分离 2 0例正常人、2 4例慢性乙型肝炎患者及 2 4例慢性重型乙型肝炎患者之PBL ,体外单独或与植物血凝素(PHA)共同培养 4 8h ,荧光素吖啶橙和溴乙啶染色PBL ,观察其凋亡细胞比例 ,采用逆转录 聚合酶链反应 (RT PCR)方法检测TRAIL、TRAIL RmRNA在PBL中的表达。结果　与正常对照组相比 ,活化诱导的淋巴细胞凋亡在慢性乙型肝炎患者明显增高 ,而在慢性重型肝炎患者则降低 ,差异有显著性 (P<0 .0 1)。PHA活化前 ,TRAILmRNA在上述 3组研究对象外周血淋巴细胞均不表达 ;PHA活化后 ,其表达明显上调 ,且慢性乙型肝炎组高于正常对照组 ,慢性重症肝炎组低于正常对照组 ,差异有显著性(P <0 .0 1)。 4个TRAIL R的mRNA在 3组研究对象外周血淋巴细胞均有表达 ,两两比较差异无显著性 (P >0 .0 5 )。经PHA活化后 ,TRAIL R2mRNA表达明显上调 (P <0 .0 1) ,TRAIL R3mRNA表达完全消失 ,TRAIL R1与 R4的mRNA表达无明显改变 (P >0 .0 5 )。结论　慢性乙型肝炎患者外周血淋巴细胞存在凋亡紊乱现象 ,TRAIL参与了乙型肝炎患者外周血淋巴细胞活化诱导细胞死亡 (Objective To study the mRNA expression of TRAIL and TRAIL-R in the PBL of patients with chronic hepatitis B. Methods The PBL of 20 normal controls, 24 patients with chronic hepatitis B and 24 patients with chronic severe hepatitis B were isolated and cultured with or without phytohemagglutinin(PHA 10μg/ml) for 48 hours. After incubation, the cells were harvested by centrifugation and apoptosis of PBL was studied by fluorescent staining. The mRNA expression of TRAIL and TRAIL-R was examined by reverse transcription polymerase chain reaction(RT-PCR). Results In comparison with normal controls, the apoptosis of PBL was significantly higher in patients with chronic hepatitis B (P<0.01)and lower in patients with chronic severe hepatitis B (P<0.01)after PHA stimulation in vitro. The expression of TRAIL mRNA was undetectable in the resting PBMC of three investigated groups, but it was obviously increased after PHA stimulation in vitro. In comparison with the group of normal controls, the expression of TRAIL mRNA in PBL was significantly higher in the group of patients with chronic hepatitis B(P<0.01)and lower in the group of patients with chronic severe hepatitis B(P<0.01). The expressions of four TRAIL-R mRNA were found in resting PBL of three investigated groups, but there was no significant difference among them(P>0.05). After PHA stimulation, the expression of TRAIL-R2 mRNA was significantly increased (P<0.01) and the expression of TRAIL-R3 mRNA was completely lost, no change was found in the expression of TRAIL-R1 and TRAIL-R4 mRNA (P>0.05). Conclusion The apoptotic dysfunction was existed in PBL of patients with chronic hepatitis B and TRAIL was involved in it. TRAIL mediated AICD is strictly regulated by TRAIL-R, TRAIL-R2 and TRAIL-R3 may play a key role in TRAIL mediated AICD of PBL. [

关 键 词：TRAIL受体 外周血淋巴细胞 慢性乙型肝炎患者 mRNA表达 对照组 正常 PBL AICD PHA 细胞死亡 

分 类 号：R512.62[医药卫生—内科学] R735[医药卫生—临床医学]