药用植物黄花乌头植株再生系统的建立  

Establishment of Plantlet Regeneration System of Medicinal Plant Aconitum coreanum

作　　者：徐秀泉[1] 于荣敏[2] 赵昱[3] 

机构地区：[1]江苏大学生物与环境工程学院 [2]暨南大学药学院,广州510632 [3]浙江大学药学院,杭州310031

出　　处：《中药材》2004年第11期797-799,共3页Journal of Chinese Medicinal Materials

摘　　要：目的 :通过茎尖培养实现药用植物黄花乌头Aconitumcoreanum (Levl )Rpaics的快速繁殖 ,建立黄花乌头植株再生系统。方法 :以黄花乌头茎尖为外植体 ,用不同的培养基和激素组合培养。结果 :MS培养基是不定芽分化和增殖的最佳基本培养基 ,附加 6 BA 2～ 5mg/L适合于不定芽的诱导 ,附加赤霉素 1～ 5mg/L和腺嘌呤 1～5mg/L能促进芽的生长与增殖 ,附加IBA 0 5～ 1 0mg/L适于根的诱导。结论 :在一定培养条件下 。Objective: To set up the rapid propagation and plantlet regeneration system of medicinal plant Aconitum coreanum(Levl.) Rpaics. Method: Adventitious buds and regenerated plantlet were obtained by shoot tip culture through different media and combination of plant growth regulators. Result: MS medium was the optimal medium for differentiation and proliferation of shoot tip. MS medium with 6-BA 2～5 mg/L was found to be most suitable for the induction of bud. MS with 6-BA 2mg/L+GA 3 1～5 mg/L or adenine 1～5 mg/L was best combination for the growth and proliferation of adventitious buds, and MS with IBA 0.5～1 mg/L for the induction of root. Conclusion: Tip culture may be used as a useful method to get regenerated plantlet of Aconitum coreanum under certain condition.

关 键 词：药用植物 黄花乌头 茎尖培养 植株再生 中药材 

分 类 号：R282.71[医药卫生—中药学] S567[医药卫生—中医学]