苦参碱对获得性多药耐药小鼠S_(180)肿瘤细胞基因表达产物P_(170)、LRP及TOPOⅡ表达的影响  

The Effect of Matrine on the Expression of P_(170),LRP and TOPO Ⅱ of Obtained Multi-drug Resistance of Mouse S_(180)'s Tumour Cell

作　　者：孙付军[1] 王宁[2] 李贵海[1] 王学荣[1] 李晓晶[1] 尹格平[3] 

机构地区：[1]山东省中医药研究院,济南250014 [2]山东省卫生学校,济南250014 [3]济南军区总医院,济南250031

出　　处：《中药材》2004年第11期838-840,共3页Journal of Chinese Medicinal Materials

基　　金：山东省自然基金资助项目 (编号 :Y2 0 0 2C3 7)

摘　　要：目的 :实验观察苦参碱口服给药对化疗诱导的多药耐药基因表达产物P170 、肺耐药蛋白LRP和拓扑异构酶Ⅱ的影响 ,探讨其干预多药耐药的分子生物学基础 ,以指导临床应用苦参碱干预化疗多药耐药的产生。方法 :以亚于治疗剂量的联合化疗PFC方案 ,建立小鼠S180 肿瘤细胞多药耐药模型 ,同时给予苦参碱 4周 ,流式细胞仪荧光检测P170 、LRP、TOPOⅡ。结果 :苦参碱明显降低化疗诱导后耐药细胞P170 、LRP的表达率和ROPOⅡ活性。说明苦参碱可通过对相关生物活性物质的调节 ,干预化疗诱发的肿瘤细胞多药耐药的产生。Objective: To observe the effect of matrine on P 170 expressed by multi-drug resistance gene, lung resistant protein(LRP), and boposomeras Ⅱ. Methods: By the method of less dosage of chemotherapy PFC, the mouse model of multi-drug resistance of S 180 tumour cell was set up. At the same time, matrine was administered to the mouse model for 4 weeks, and then P 170 , LRP, TOPOⅡ were observed by flow cytometry. Results: Martine can obviously reduce the express of P 170 , LRP and the activity of TOPOⅡ in multi-drug resistance tumour cell induced by chemotherapy. Conclusion: Matrine can intervene the occurrence of the multi-drug resitance of tumor cells induced by chemotherapy.

关 键 词：苦参碱 获得性多药耐药 小鼠 S180 肿瘤细胞 P170 LRP 基因表达 中医药疗法 拓扑异构酶Ⅱ 

分 类 号：R273[医药卫生—中西医结合] R285.5[医药卫生—中医肿瘤科]