眼镜蛇毒组份在动物体内外的抗氧化作用研究  

Effect of Fractions Isolated from Naja naja atra Venom on Antioxidation in Animals

作　　者：侯力强[1] 何泉华[1] 赵路宁[1] 孔天翰[1] 管锦霞[1] 江中喜[2] 

机构地区：[1]广州医学院蛇毒研究所,广州510182 [2]广州医学院第二附属医院,广州510260

出　　处：《中药材》2004年第11期845-848,共4页Journal of Chinese Medicinal Materials

基　　金：广东省医学科学技术研究基金 (课题号B19990 75;B2 0 0 0 0 67);广东省自然科学基金博士启动基金(课题号 9944 0 9)资助

摘　　要：目的 :以大鼠组织匀浆及红细胞为材料 ,研究中华眼镜蛇毒组份F和H的抗脂质过氧化作用 ;探讨组份F/H对小鼠体内抗氧化酶活性的影响。方法 :以MDA为指标 ,观察组份F/H对组织匀浆体外脂质过氧化及红细胞自氧化溶血体系脂质过氧化产物MDA生成量的影响 ;观察组份F/H对邻苯三酚自氧化产生的O ·2 及Cu2 + VitC自由基产生系统产生的·OH的清除作用 ;连续 15天腹腔注射组份F/H ,测定小鼠体内SOD、CAT的活性。结果 :组份F/H均能抑制红细胞自氧化 ,显著减少红细胞自氧化过程中MDA的含量 ;组份F/H均可抑制正常大鼠心、肝、脑组织匀浆体外过氧化脂质生成 ,并能对抗Fe2 + Cys激发的过氧化脂质生成增加 ;组份F/H均能清除超氧阴离子 (O ·2 )和羟自由基 (·OH) ,组份H的作用更强 ;组份F/H均能不同程度地增加小鼠体内多种抗氧化酶的含量。结论 :组份F和H具有一定的抗氧化作用 ,能直接清除活性氧自由基 ,提高SOD的活性。Objective:To study the antioxidation of fraction F and H isolated from Naja naja atra venom on homogenate and RBC autoxidation. And to explore the effect of two fractions on the activities of antioxidation enzymes in mice.Method:Samples of tissues homogenates and RBC suspension were pretreated with fraction F/H,and then their generation of malondialdehyde(MDA) was examined. The effects of fraction F/H on O -· 2 produced by pyrogallol autoxidation and ·OH produced by Cu 2+ -VitC were checked. After being administrated i.p.with fraction F/H for 15 days,superoxide dismutase and hydroperoxidase in mice were measured for their activities.Result:Fraction F/H inhibited RBC autohemolysis and the generation of MDA decreased. The production of lipid peroxides in normal brain,liver and heart homogenate of rat and the elevation of lipid peroxides induced by cysteine and FeSO 4 in homogenate were inhibited by addition of fraction F/H. The O -· 2 produced by autoxidation of pyrogallol and the ·OH produced by Cu 2+ -VitC system could be scavenged by fraction F/H(the potency of scavenging oxygen free radical is that fraction H> fraction F). Fraction F/H all increased the activities of several antioxidation enzymes in mice to different extents.Conclusion:Fraction F/H have the effect of antilipid peroxidation,may scavenge active oxygen free radical and increase the activity of SOD.

关 键 词：眼镜蛇毒 抗氧化作用 抗氧化酶 氧自由基 脂质过氧化 

分 类 号：R996.3[医药卫生—毒理学]