短肠综合征病人血清氨基酸谱的测定  

The measurement of serum amino acids in short bowel syndrome patients

作　　者：罗楠[1] 刘放南[1] 谭力[1] 蒋小华[1] 朱维铭[1] 李宁[1] 

机构地区：[1]南京军区南京总医院解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2004年第6期367-368,372,共3页Parenteral & Enteral Nutrition

摘　　要：目的 :用高效液相色谱法检测短肠综合征病人的血清氨基酸谱。　方法 :共测定 17例短肠综合征病人的血清氨基酸谱 ,并以 15例正常人空腹血作为对照 ,将异硫氰酸苯酯 (PITC)作为柱前衍生剂 ,用甲醇、乙腈和乙酸钠作为二元梯度洗脱 ,6 7min完成分离。　结果 :17例短肠综合征病人血清缬氨酸、丝氨酸、胱氨酸非常显著低于正常人 (P<0 .0 1) ,血清亮氨酸、异亮氨酸、赖氨酸、蛋氨酸、组氨酸显著低于正常人 (P <0 .0 5 )。　结论 :用高效液相色谱法检测氨基酸稳定可靠 ,短肠综合征病人存在氨基酸特别是必需氨基酸的缺乏。Objective: To observe serum amino acids levels in patients with short bowel syndrome(SBS) by high performance liquid chromatography(HPLC). Methods: The serum samples of 17 SBS patients were collected, and 15 healthy volunteers were as the control group .PITC was used as pre-column derivative of HPLC. Two phases gradient elution was performed with methanol and acetic sodium for 67 minutes . Results: The serum levels of Val,Ser and Cys were significantly lower in SBS patients than that of healthy volunteers(P<0.01).The serum levels of Leu,Ile,Lys,Met and His in SBS patients were lower too (P<0.05). Conclusions: The HPLC method to detect the serum amino acids is steady and reliable. Most of amino acids, especially the essential amino acids,are greatly lacked in SBS patients.

关 键 词：短肠综合征 血清氨基酸谱 测定 高效液相色谱法 

分 类 号：R656.7[医药卫生—外科学]