一种改良的精斑涂片DNA检验方法  

A sort of Meliorative Method for Testing DNA from Sperm Smear

作　　者：孙耀东 任宏 陈月勇 

机构地区：[1]盐城市公安局刑事科学技术研究所,江苏盐城224002

出　　处：《法医学杂志》2004年第4期202-204,共3页Journal of Forensic Medicine

摘　　要：目的建立一种有效的精斑涂片DNA检验方法。方法以本实验室平时积累的91例精斑涂片为研究对象,采用chelex法提取,结合硅珠法纯化浓缩,提高DNA浓度后进行PCR扩增和STR分型。结果尽管涂片上精子量较少,但chelex法的DNA提取率较高,且硅珠法可以纯化并浓缩模板DNA,本文建立的方法兼具了这两种提取方法的优点,成功率较高。结论精斑涂片可以作DNA检验,在法医检案中具有较高的应用价值。Objective To establish an effective method for testing DNA from the sperm smear. Methods We did exploratory research for ninety-one cases of the sperm smear which were accumulated by our laboratory during working hours. Chelex extraction method was used to extract DNA.The digested solution of sperm was purified and concentrated by using Silicon bead. STR loci were typed after PCR amplification by Profile Plus kit. Results Although there were a few sperm cells on the slide,it is easy to obtain good DNA typing result from it because the amalgamative method we used was high effective for DNA extraction. Conclusion The DNA analysis of sperm smear could offer satisfactory typing results which would be useful in case.

关 键 词：精斑涂片 DNA检验 STR分型 法医物证检验学 

分 类 号：D919.2[医药卫生—法医学]