淋巴细胞活化在系统性红斑狼疮发病中的意义  

Significance of lymphocytes activation in pathogenesis of systemic lupus erythematosus

作　　者：肖东杰[1] 刘洋[1] 黄平[1] 李晓英[2] 杨博[1] 汪运山[1] 

机构地区：[1]济南市中心医院生物治疗中心,250013 [2]山东中医药大学附属医院

出　　处：《中国麻风皮肤病杂志》2004年第6期528-530,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的 :　探讨系统性红斑狼疮 (SLE)患者外周血淋巴细胞HLA -DR以及植物凝集素 (PHA)刺激下的CD3+ CD4 + 、CD3+ CD8+ 、CD19+ 淋巴细胞的早期活化标志CD6 9的表达。方法 :　应用三色荧光标记流式细胞术检测活动期和非活动期SLE患者外周血CD3+ 、CD3+ CD4 + 、CD3+ CD8+ 淋巴细胞晚期活化标志HLA -DR以及在PHA刺激 4h后CD3+ CD4 + 、CD3+ CD8+ 、CD19+ 淋巴细胞的早期活化标志CD6 9的表达。结果 :　①PHA刺激 4小时后 ,活动期SLECD3+ CD4 + 、CD3+ CD8+ 细胞CD6 9的表达明显低于正常对照组 (P <0 .0 5 ) ;而非活动期SLE与正常对照组之间CD6 9表达无显著性差异 (P >0 .0 5 )。②PHA刺激前SLE活动组CD19+ 细胞表达CD6 9较正常对照组和SLE非活动期明显升高 (P <0 .0 5 ) ,PHA刺激 4小时后 ,活动期SLECD19+ 细胞表达CD6 9低于正常对照组和非活动期SLE ,存在显著性差异 (P <0 .0 5 ) ,而非活动期SLE与正常对照组之间CD19+ CD6 9+ 表达无明显差异 (P >0 .0 5 )。③活动期SLECD3+ HLA -DR+细胞明显高于正常对照组 (P <0 .0 1)和非活动期SLE(P <0 .0 1) ,CD3+ CD4 + HLA -DR+ 细胞、CD3+ CD8+HLA -DR+ 细胞明显高于正常对照组 (P <0 .0 5 ) ,而非活动期SLE与正常对照组之间 ,HLA -DR表达无显著性差异 (P >0 .0Objective: To measure the expression of HLA-DR-a later activated marker on lymphocytes and the expression of CD69-a early activated marker on CD3+CD4+, CD3+CD8+, CD19+ in peripheral blood lymphocytes(PBL) after stimulated by phytohaemagglutinin (PHA) in patients with active or inactive systemic lupus erythematosus (SLE). Methods: The expression of HLA-DR on CD3+, CD3+CD4+, CD3+CD8+ lymphocytes and of CD69 on CD3+CD4+,CD3+CD8+,CD19+ lymphocytes in PBL after 4 hours stimulated by PHA in patients with active or inactive SLE was measured with tri-color-fluorescence cytometry. Results: The expression of CD69 on CD3+CD4+,CD3+CD8+ cells was lower in active patients than that in control group after 4 hours stimulated by PHA (P<0.05), but the expression of CD69 of inactive patients was not different from the normal group (P>0.05). The expression of CD69 on CD19+ cells in active patients was higher than those in control group and in inactive patients before stimulated by PHA (P<0.05), but the expression was lower in active patients than both in control group and in inactive patients (P<0.05) after 4 hours stimulated by PHA (P<0.05). The expression of CD69 on CD19+ cells of inactive patients was not significantly different from that in control group (P>0.05). In active patients of SLE, the CD3+HLA-DR+ cells were higher than that of control group (P<0.01) and that of inactive patients (P<0.01). The CD3+CD4+HLA-DR+ and CD3+CD8+HLA-DR+ cells were higher than that of control group (P<0.05), but the expression of HLA-DR in inactive patients was not significantly different from that in normal group (P>0.05). Conclusion: In active patients of SLE,T and B cells in PBL are abnormally activated, and the early reaction of these cells to stimulation is decreased. These changes may be associated with pathogenesis of SLE.

关 键 词：淋巴细胞 细胞活化 系统性红斑狼疮 SLE CD3 CD4 CD8 CDL9 HLA-DR CD69 

分 类 号：R593.241[医药卫生—内科学]