肾缺血预处理对未成熟心肌凋亡蛋白表达的影响  

The effects of renal ischemic preconditioning on the expression of immature myocardial cell apoptotic proteins

作　　者：孙忠东[1] 宋玉娥[2] 池一凡[1] 杨铁南[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院心外科,山东青岛266011 [2]青岛大学医学院附属青岛市立医院门诊部,山东青岛266011

出　　处：《心脏杂志》2004年第6期520-522,共3页Chinese Heart Journal

摘　　要：目的 :探讨肾缺血预处理 (RIP)对未成熟心肌细胞凋亡相关蛋白表达的影响。方法 :采用兔Langendorff模型 ,幼兔 2 4只随机分为 3组 ,对照组 (C ,n =8) :仅灌注KH液 180min ,然后取标本 ;缺血 /再灌组 (I/R ,n =8) :灌注 2 0min后 ,停灌 4 5min ,再灌 12 0min ;肾缺血预处理组 (RIP ,n =8) ,反复 2次阻断肾血流 5min/放开 5min ,建立Langendorff模型 ,然后重复I/R组缺血 /再灌方法。以凋亡细胞原位标记与半定量分析、琼脂糖凝胶电泳检测DNA片段梯及Bcl 2、Bax、Fas基因蛋白的表达作为观察指标。结果 :凋亡细胞原位标记与半定量分析 :RIP组与I/R组比较 ,心肌细胞凋亡率显著减少 ;琼脂糖凝胶电泳检测DNA片段梯 :RIP组与I/R组比较 ,光密度显著降低 ;RIP组与I/R组比较 ,Bcl 2表达明显增多 ,Bax、Fas表达显著减少。结论 :RIP可能通过调节Bcl 2、Bax、Fas蛋白的不同表达影响心肌细胞的凋亡。AIM: To investigate the effects of renal ischemic preconditioning (RIP) on immature myocardial cell apoptosis and expressions of Bcl-2,Bax and Fas proteins.METHODS: Isolated rabbit heart Langendorff model was used in this study. 24 immature rabbits (aged 14～21 days) were randomly divided into 3 groups. The control group were only perfused for 180 minutes. The ischemia/reperfusion group were perfused 20 minutes,and left with myocardial ischemia for 45 minutes and then followed by reperfusedion for 120 minutes. The right kidney arteries of the RIP group were clamped 5 minutes and then followed by 5 minutes reperfusion 2 times,and then by repeating the method of I/R group. The terminal transferase UTP nick end labeling(TUNEL), nucleosomal ladder of DNA fragments, and the expression of Bcl-2, Bax,and Fas protein were detected. RESULTS: There were marked reduction in TUNEL cell apoptosis,ladder of DNA fragments,expressions of Bax and Fas protein,and marked increase in expression of Bcl-2 protein in the RIP group,compared with the I/R group. CONCLUSION: This study demonstrated that RIP decrease immature myocardial cell apoptosis by regulating the expressions of Bcl-2, Bax,and Fas protein.

关 键 词：肾缺血预处理 细胞凋亡 凋亡蛋白 

分 类 号：R331.31[医药卫生—人体生理学] R542.22[医药卫生—基础医学]