rhEPO、rhG-CSF对芥子气全身吸收中毒犬疗效观察  

Therapeutic effects of rhEPO, rhG-CSF on sulfur mustard induced toxicity in dogs

作　　者：蔡芸[1] 马骞[1] 张黎明[1] 赵杰[1] 朱明学[1] 胡卫江[2] 江鹏亮[2] 袁卫[2] 

机构地区：[1]第二军医大学海医系防化医学教研室,上海200433 [2]第二军医大学实验动物中心

出　　处：《卫生研究》2004年第6期649-651,共3页Journal of Hygiene Research

基　　金：全军医药卫生"十五"指令性课题 (No .0 1L054)

摘　　要：目的　评价重组人红细胞生成素 (rhEPO)、重组人粒细胞集落刺激因子 (rhG CSF)对芥子气全身吸收中毒犬的治疗效果。方法　以成年家犬为中毒模型 ,共 10条 ,中毒剂量为 12mg kg,皮下注射。随机分为中毒对照组 4条 ,治疗组 6条 ,治疗组动物在中毒后 30min即进行相应的药物治疗。中毒前所有犬各测一次血常规的基础值 ,中毒后每日监测血常规的动态变化 ,持续 1周。结果　对照组犬于 3天内死亡 3条 ,治疗组犬全部成活。对照组白细胞计数 (WBC)在中毒后第 3开始剧烈下降 ,红细胞计数 (RBC)在中毒后即有轻度上升 ,之后回落 ;而治疗组WBC、RBC在中毒后 1周内均无显著变化。两组的淋巴细胞计数 (LYM)在中毒后都有显著下降 ,治疗组稍好于对照组。治疗组的网织红细胞计数 (RC)于中毒后第 2天开始显著增长 ,之后维持在高水平 1周左右。治疗组的RC变化不明显。结论　犬芥子气中毒后 ,应用rhEPO、rhGObjective To evaluate the therapeutic effects of recombinant human erythropoietin (rhEPO) and recombinant human granulocyte colony stimulating factor (rhG CSF) on sulfur mustard (SM) induced toxicity in dogs. Methods Ten dogs were used as the animal model. Control group had four dogs while treatment group had six. Half an hour after subcutaneously injected with 12mg/kg (body weight) of SM, the dogs of the treatment group were treated by rhEPO, rhG CSF and other medicines. General examinations of blood were measured before injecting SM and were continually watched for a week after poisoning. Results Three dogs in control group died in three days after poisoning, dogs in treatment group all survived. WBC of the control group decreased significantly 3 days after poisoning. RBC of the control group had a slight increase at first, then came down. While WBC and RBC of treatment group remained constant. LYM of both groups descended notably right after poisoning, especially in control group. Two days after poisoning, RC in treatment group began to rise and remained at high level for about a week. No obvious changes of RC in control group were found. Conclusion The use of rhEPO and rhG CSF after SM exposure can stimulate the growth of the erythrocyte, reticulocyte and leucocyte in dogs. Therefore, rhEPO and rhG CSF may be significant assistant drugs in future experiment of SM.

关 键 词：芥子气 中毒 重组人红细胞生成素(rhEPO) 重组人粒细胞集落刺激因子(rhG-CSF) 

分 类 号：R827.176[医药卫生—临床医学] R595.1