利用蛋白质组学技术研究氢醌刺激后人胚肺成纤维细胞蛋白质表达改变  

Protein changes in human embryonic lung fibroblasts after hydroquinone stimulation using proteomic technique

作　　者：李习艺[1] 庄志雄[1] 刘建军[1] 黄海燕[1] 卫秦芝[1] 杨晓华[1] 

机构地区：[1]中山大学公共卫生学院,广州510080

出　　处：《卫生研究》2004年第6期654-657,共4页Journal of Hygiene Research

基　　金：国家 973计划资助项目 (No .2 0 0 2CB51 2 90 4 )

摘　　要：目的　研究氢醌 (HQ)对人胚肺成纤维细胞 (HLF)蛋白质表达的影响 ,以阐明氢醌引起细胞应答反应的分子机制。方法　细胞受HQ刺激后 ,加入裂解液 ,取上清丙酮沉淀 ,蛋白定量。双向凝胶电泳分离蛋白质组成分 ,胶体考马斯亮兰显色 ,Imagemaster2 0进行图像分析后选取差异点 ,胶内酶解后MALDI TOF进行肽指纹图谱鉴定。结果　图像分析结果显示 ,对照组HLF细胞检测到 4 90± 2 8个蛋白点 ,HQ染毒组为 4 38±2 3个。氢醌刺激后 15个蛋白斑点发生变化 ,初步鉴定了其中 8个蛋白斑点 ,包括一些氧化应激和细胞骨架相关蛋白。结论　氢醌可引起HLF细胞蛋白表达改变。Objective To explore the molecular mechanism of cell response induced by hydroquinone, we investigated the protein profile after treatment with hydroquinone in human embryonic lung fibroblasts. Methods After HQ treatment, cells were lysed in lysis solution, then the supernatant were collected and precipitated with acetone prior to protein concentration determination. The total cellular proteins were separated using two-dimensional gel electrophoresis and visualized by colloidal coomassie blue staining. Digital images were analyzed using Imagemaste 2.0 software. The differentially expressed protein spots were picked and digestion in gel then identified by peptide mass fingerprinting using MALDI-TOF. Results There were 15 protein spots changed after HQ stimulation. Among them 8 protein spots were identified by PMF including some oxidative stress and cytoskeleton related proteins. Conclusion Protein profile was altered after HQ stimulation.

关 键 词：氢醌蛋白质组 细胞应答 细胞骨架 氧化应激 

分 类 号：R994.4[医药卫生—毒理学] Q51[医药卫生—药学]