NQO1基因多态性与慢性苯中毒遗传易感性的研究  

Study on genetic polymorphism of NQO1 and susceptibili ty to benzene poisoning

作　　者：陈艳[1] 纪之莹[1] 许建宁[1] 吴春玲[1] 李桂兰[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《卫生研究》2004年第6期657-659,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助 (No .39870 685)

摘　　要：目的　探讨NQO1基因多态性与慢性苯中毒遗传易感性之间的关系。方法　选择 10 0名慢性苯中毒工人为病例组及 90名同期接苯但无苯中毒表现的同工种工人为对照组 ,应用PCR RFLP方法判定NQO1基因型。结果　携带NQ0 1C6 0 9TT T基因型 (纯合突变型 )个体发生苯中毒的危险性是具有C T基因型 (杂合型 )和C C基因型 (野生型 )个体的 2 82倍 (95 %CI:1 4 2～ 5 5 8) ,是具有C C基因型 (野生型 )个体的 2 94倍 (95 %CI:1 2 5 - 6 90 ) ;并存在携带NQO1C6 0 9TT T基因型 (纯合突变型 )个体发生苯中毒的危险性高于携带NQO1C6 0 9TC T基因型 (杂合型 )个体、更高于C C基因型 (野生型 )个体的趋势 (χ2trend=6 0 1,P =0 0 14 )。结论　携带NQO1C6 0 9T纯合突变基因型 (T T)个体接苯时发生苯中毒的危险性增高 ,考虑此基因可作为易感性生物标志物 。Objective To explore the relationship between genetic polymorphism of NQO1 and susceptibility to benzene poisoning (BP).Methods The genetic polymorphism of NQO1 for 100 patients with benzene poisoning and 90 workers exposed to benzene who were engaged in the same working time and job title as patients with benzene poisoning were detected by PCR-RFLP. Results There was a 2.82-fold (95% CI:1.42-5.58 ) increased risk of BP in the subjects with NQO1 C609T mutation genotype(T/T) compared with those carrying heterozygous (C/T) and wild type (C/C), and there was a 2.94-fold (95%CI:1.25-6.90) increased risk of BP in the subjects with NQO1 C609T mutation genotype(T/T) compared with those carrying wild type (C/C). There was a trend that the risk of BP in subjects with NQO1 C609T mutation genotype(T/T) was higher than those carrying heterozygous (C/T) and even higher than those carrying wild type (C/C) (χ 2 trend =6.01, P=0.014). Conclusion The subjects with NQO1 C609T mutation genotype(T/T) were more susceptive to benzene and NQO1 is as a biomarker to assessment the risk of benzene poisoning for individual.

关 键 词：NQ01慢性苯中毒基因多态 遗传易感性 

分 类 号：R135.12[医药卫生—劳动卫生] Q78[医药卫生—公共卫生与预防医学]