p,p′-DDE对离体培养的大鼠睾丸支持细胞MAPK通路的影响  

Effects of p,p′-DDE on intracellular MAPK pathway of rat sertoli cells in vitro

作　　者：王翀[1] 梁娇君[1] 王爱国[1] 杨克敌[1] 

机构地区：[1]华中科技大学同济医学院,武汉430030

出　　处：《卫生研究》2004年第6期668-670,共3页Journal of Hygiene Research

基　　金：国家 973项目资助 (No .2 0 0 2CB51 2 90 8)

摘　　要：目的　研究p ,p′ DDE对离体培养大鼠睾丸支持细胞内信号转导通路的影响。方法　从大鼠睾丸组织中分离支持细胞进行离体原代培养 ,以SABC方法来检测细胞内p ERK的表达。结果　发现支持细胞细胞内p ERK随p,p′ DDE剂量的增高而染色加深 ,而且胞浆胞核都表达。结论　p ,p′ DDE可导致支持细胞内ERK磷酸化 ,诱导ERK下游通路的激活 ,从而影响到基因的转录和表达。Objective To study effects of p,p′-DDE on intracellular p-ERK of rat sertoli cells in vitro. Methods We cultulred sertoli cells separated from testicular tissue of rats and examined the changes of intracellular p-ERK, using immunohistochemical SABC method. Results It was showed that intracellular p-ERK increased with the dose of p,p′-DDE and had a dose-dependent trend. Conclusion The results suggested that p,p′-DDE could result in the phosphorylation of ERK and induce the activation of the downstream road and the transcription and expression of genes.

关 键 词：支持细胞 细胞内p^-ERK P p′-DDE 

分 类 号：R994.6[医药卫生—毒理学] Q756[医药卫生—药学]