有机锗多酸衍生物抗呼吸道病毒体外试验  

Study on Vitro Anti-respiratory Virus Infection Effect of Organogermanium Poly-derivatives

作　　者：田丽丽[1] 张桂英[1] 王宝贵[1] 赵林伊[1] 袁振丽[1] 潘晓锐[2] 

机构地区：[1]吉林大学公共卫生学院,吉林长春130021 [2]吉林省人民医院,吉林长春130021

出　　处：《癌变．畸变．突变》2004年第6期349-351,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:探讨有机锗多酸衍生物体外毒性及其在体外对呼吸道合胞病毒、腺病毒和流感病毒抑制作用,为临床应用提供科学依据。材料与方法:建立体外病毒感染模型,通过改变给药时间、途径和剂量,采用细胞病变法(CPE)和MTT法观察有机锗多酸衍生物对呼吸道病毒感染细胞的保护作用。结果:有机锗多酸衍生物在160.00、80.00、40.00 μg/ml时,先感染病毒后加药、感染病毒同时加药和感染病毒后加药3种加药方式下细胞存活量均高于病毒对照组。有机锗多酸衍生物在3种加药方式下的高剂量组和先感染病毒再加药方式下的高、中、低剂量组,对感染100TCID50 RSV单层Hela细胞的保护作用高于利巴韦林对照组。结论:有机锗多酸衍生物具有抗呼吸道病毒作用。BACKGROUND & AIM: To study the cytotoxicity on normal cell and antiviral effect of organogermaniurn Poly-derivatives on breath virus, and provide a basis to clinical application. MATERIAL AND METHODS: The cytoxicity on FL was studied with the method of MTT. Establish the model of breath viral infected in vitro and observe protective function of organogermaniurn poly-derivatives on infected cells by methods of CPE and MTT. To study the antiviral effect of organogermanium poly-derivatives by changing time, way and concentration of giving medicine. RESULTS: The quantity of surviving cells was more than virus infecting group. When the dosage reached 160.00 μg/ml, inhibiting RSV effect of organogermanium poly-derivatives was higher than the ribavirin group.In the group that the Hela cells were infected by RSV first then treated with organogermanium poly-derivatives, the quantity of alive cell was more than the ribavirin group. CONCLUSION: organogermanium poly-derivatives is a kind of medicine that has definite antiviral function.

关 键 词：有机锗 多酸衍生物 抗病毒 细胞病变 

分 类 号：R284.1[医药卫生—中药学] R972[医药卫生—中医学]