硝酸羟胺对雄性小鼠生殖细胞和精子影响的初步研究  

Effect on Testicular Germ Cell and Sperm in Mouse After Exposure to Hydroxylammonium Nitrate

作　　者：贾庆军[1] 杨录军[1] 刘晋祎[1] 周燕虹[1] 刘胜学[1] 周紫桓[1] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理教研室,重庆400038

出　　处：《癌变．畸变．突变》2004年第6期362-364,371,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：军队指令性课题(No.180.3160-O1L015);国家杰出青年基金资助项目(No.30125037);军队杰出青年基金资助项目(No.01J014)

摘　　要：背景与目的:探讨不同剂量的硝酸羟胺对小鼠睾丸生殖细胞微核、精子畸形以及睾丸生殖细胞DNA的影响。材料与方法:小鼠腹腔注射不同浓度的硝酸羟胺溶液,小鼠生殖细胞微核试验连续4 d染毒,第5 d取双侧睾丸;小鼠精子畸形试验、睾丸生殖细胞DNA流式细胞仪分析连续5 d染毒,染毒后28 d取双侧附睾、睾丸,计数小鼠精子畸形率及生殖细胞DNA分布情况,评价硝酸羟胺对小鼠的生殖毒性作用。结果:腹腔注射硝酸羟胺实验组生殖细胞微核率、精子畸形率与对照组相比差异均有显著性;睾丸生殖细胞流式仪分析中,在高剂量组圆形、长形精子细胞DNA百分率与对照组相比差异有显著性。结论:小鼠腹腔注射硝酸羟胺对雄性小鼠生殖细胞和精子有损害作用。BACKGROUND & AIM: To study the reproductive damage of testicular germ cell and the deformity of sperm in mouse induced by Hydroxylammonium Nitrate (HAN) . MATERIAL AND METHODS: Testicular germ cell micronucleus test, sperm deformity assay and flow cytometry were used. RESULTS: It showed that the percentage of deformity in sperm was markedly increaed as the dose increased(P < 0.05), the percentage of micronucleus was significately decreased as the dose increased (P < 0.05) . The toxic effect of sperm was correlated with exposuring HAN . CONCLUSION: It suggested that HAN might have reproductive damage effect to testicular germ cell and sperm in mice.

关 键 词：硝酸羟胺 睾丸生殖细胞 精子 流式细胞术 

分 类 号：R994.6[医药卫生—毒理学]