硝酸羟胺诱导小鼠淋巴瘤细胞tk基因突变的研究  

Studies of tk Gene Mutation by Hydroxylammonium Nitrate

作　　者：贾庆军[1] 刘胜学[1] 刘晋祎[1] 杨录军[1] 周燕虹[1] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理教研室,重庆400038

出　　处：《癌变．畸变．突变》2004年第6期365-367,371,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：军队指令性课题(No.180.3160-O1L015);国家杰出青年基金资助项目(No.30125037);军队杰出青年基金资助项目(No.01J014)

摘　　要：背景与目的:研究硝酸羟胺对小鼠淋巴瘤L5178Y-3.7.2c tk+/-细胞tk基因突变的影响。材料与方法:使用硝酸羟胺对L5178Y细胞进行梯度染毒,再分别进行细胞毒性,细胞接种效率,相对存活率,相对悬浮生长率和突变频率测定。结果:随着硝酸羟胺的剂量增加,L5178Y细胞的相对存活率和悬浮生长率均下降。在硝酸羟胺50-500 μg/ml范围内可以诱导细胞琥基因的突变率达到自发突变率几倍至十几倍。结论:硝酸羟胺对L5178Y细胞具有明显的细胞毒性,并可以诱导tk基因突变。BACKGROUND & AIM: To investigate the effects of Hydroxylammonium Nitrate (HAN) on the tk gene of mouse lymphoma cells. MATERIAL AND METHODS: L5178Y cells were treated with Hydroxylammonium Nitrate with different concentrations, to determine cytotxicity and mutation at different time phases. The numbers of positive wells were counted . The cell plating efficiency, relative suspension growth and mutation frequency were determined. RESULTS: The results showed that HAN (50-500 μg/ ml) induced tk gene mutation with mutation frequency 1 - 19 times higher than that of spontaneous mutation frequency of L5178Y cells and severe cytotoxic responses. CONCLUSION: Hydroxylammonium Nitrate can exert cytotocicity and mutagenic effects on tk geng in L5178Y cells.

关 键 词：硝酸羟胺 tk位点 致突变作用 L5178Y细胞 

分 类 号：R994.6[医药卫生—毒理学]