硝酸羟胺亚慢性染毒对大鼠骨髓嗜多染红细胞微核率的影响  

Effect of Exposure to Hydroxylammonium Nitrate on Micronucleus Frequency of Bone Marrow Cells in Wistar Rat for 90 Days

作　　者：安辉[1] 刘晋祎[1] 杨录军[1] 刘胜学[1] 周燕虹[1] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理教研室,重庆400038

出　　处：《癌变．畸变．突变》2004年第6期372-374,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：军队指令性课题(180.3160-O1L015);国家杰出青年基金资助项目(30125037);军队杰出青年基金资助项目(01J014)

摘　　要：背景与目的:研究硝酸羟胺亚慢性染毒对大鼠骨髓嗜多染红细胞微核率的影响。材料与方法:设置硝酸羟胺6.97、13.93、27.86 mg/kg3个不同剂量组和生理盐水阴性对照组,间日腹腔注射大鼠,连续染毒90 d后脱颈椎处死2/3实验动物;剩余1/3实验动物停止染毒再饲养30 d后同法处死;观察并统计大鼠骨髓嗜多染红细胞的微核率。结果:硝酸羟胺27.86 mg/kg组诱导的大鼠骨髓嗜多染红细胞微核率与阴性对照组、6.97 mg/kg组相比差异均具有显著性;6.97和13.93 mg/kg组与阴性对照组比较差异无显著性;而恢复期各染毒组与阴性对照组相比差异均无显著性。结论:硝酸羟胺亚慢性染毒对大鼠具有一定的遗传毒性,在一定的时间内该毒性可能具有可逆性但无延迟性。BACKGROUND & AIM: To study the effect of exposure to hydroxylammonium nitrate (HAN) on micronucleus(MN) frequency of polychromatic erythrocytes(PCE) from Wistar rats marrow. METERIAL AND METHODS: Different doses of HAN at 6.97 mgAg, 13.93 mg/kg, 27.86 mgAg were administered to the three treated groups by intraperitoneal injection,and the negative control group was treated with 0.9 % NaCl. The treatment had been performed every other day for 90 days. After the last treatment in 90 th day, 2/3 of the rats were sacrificed by cervical dislocation, and after 30 days' recovery we sacrificed the rests. The number of micronucleus per 1 000 polychromatic erythrocytes (PCE) from sternum bone marrow was counted and analysed. RESULTS: In the period of exposure to HAN, the MN frequency of bone marrow cells of Wistar rats treated by HAN wasn' t significantly different from that of the negative control group, except for the highest dose group. After 30 days' recovery, the MN frequency treated by HAN was not significantly different from that of the control group. CONCLUSION: Our results suggested that HAN can produces certain genotoxicity,which was reversible but didn't have delayed effect.

关 键 词：硝酸羟胺 微核 遗传毒性 大鼠 

分 类 号：R994.6[医药卫生—毒理学]