抗氧化剂与钙离子拮抗剂联合应用预防大鼠睾丸纤维化实验研究  

Experimental Study on Antioxidant and Calcium Channel Blockade Preventing Testicular Fibrosis in Rats

作　　者：郭洪胜[1] 安长新[2] 甘子明[1] 买尔江[1] 刑绥光[1] 陈荣[1] 

机构地区：[1]新疆医科大学组织胚胎学教研室,新疆乌鲁木齐830054 [2]广东医学院组织胚胎学教研室,广东湛江524023

出　　处：《中华男科学杂志》2004年第11期857-863,866,共8页National Journal of Andrology

摘　　要：目的 :研究抗氧化剂与钙离子拮抗剂联合应用对大鼠睾丸纤维化的防治效果 ,以探索防治睾丸纤维化发生的理想药物。　方法 :将正常雄性Wistar大鼠 80只分为正常对照组 (n =10 ) ,高 (n =2 0 )、中 (n =2 0 )、低 (n =17)预防睾丸纤维化组和睾丸纤维化模型组 (n =13) ,采用王涛等建立的大鼠睾丸纤维化模型的方法略加改进。从第 1次免疫的次日起 ,高剂量组给予维生素E、C合剂 90mg/ (kg·d)、维拉帕米 5 0mg/ (kg·d)灌胃 ,中剂量组给予维生素E、C合剂 90mg/ (kg·d)、维拉帕米 2 5mg/ (kg·d)灌胃 ,低剂量组给予维生素E、C合剂 90mg/ (kg·d)、维拉帕米 12 .5mg/ (kg·d)灌胃 ,共 15 0d。纤维化模型组未予干预措施。正常对照组未加任何处理。检测精子计数、精子畸形率、睾丸长度、精曲小管直径 ,光镜和透射电镜观察睾丸间质及精曲小管生精细胞的变化。　结果 :高、中剂量组对于预防大鼠睾丸纤维化效果显著 ,睾丸精曲小管直径、基膜厚度与正常对照组相比差异无显著性。低剂量组精曲小管界膜略增厚 ,生精细胞损伤较重 ,但病变仍轻于模型组。模型组睾丸间质增生显著 ,间质中、精曲小管的管周及睾丸被膜下可见肥大细胞增多 ,精曲小管的界膜显著增厚 ,生精上皮空泡变。　结论 :抗氧化剂与钙离子拮抗剂联合应用 ,Objective: To investigate the preventive effect of antioxidant and calcium channel blockade on testicular fibrosis in rats, and to explore the ideal drug for preventing it. Methods: Eighty Wistar rats were divided into a control group(Group A, n=10), a treatment group (Group B, n=57) and a testicular fibrosis model group (Group C, n=13). And the treatment group was further divided into a higher dosage group ( Group a, n=20), a medium dosage group (Group b, n=20) and a lower dosage group( Group c, n=17). Testicular fibrosis was duplicated with altered Wang Tao's method. From the second day of the first immunization, the higher dosage group was given antioxidant vitamins 90 mg/(kg·d)and verapamil 50 mg/(kg·d), the medium dosage group antioxidant vitamins 90 mg/(kg·d)and verapamil 25 mg/(kg·d), and the lower dosage group antioxidant vitamins 90 mg/(kg·d) and verapamil 12.5 mg/(kg·d), all for 150 days. The control and the model groups received no treatment. The sperm count, sperm deformity rate, testis length and seminiferous tubule intradiameter were measured, and the changes of the testis interstitial substance and spermatogenic cells were observed by light microscope and transmission electron microscope. Results: Testicular fibrosis was significantly prevented by the higher- and medium-dosage treatment in the rats. In the higher dosage group, the intradiameter of the seminiferous tubules and the thickness of the limiting membrane were almost the same as in the control. In the lower dosage group the thickness of the limiting membrane was thicker and the damage to the spermatogenic cells was heavier than in the control, but the patholigical changes of the testis structure was lighter than in the model group, in which Hyperplasia and fibroblast increase in the interstitial substance were significant, interstitial mast cells and peritubular mast cells increased, the thickness of the limiting membrane of the seminiferous tubules seriously thickened, and the damage to the spermatogenic cells was severe. Co

关 键 词：睾丸疾病 纤维化 维生素E 维生素C 维拉帕米 大鼠 

分 类 号：R96[医药卫生—药理学]