葛根素对心肌缺血-再灌注损伤兔乳酸脱氢酶活性变化的影响  

Influence of puerarin on the change of lactic dehydrogenase activity in myocardial ischemia/reperfusion injury in rabbits

作　　者：王万铁[1] 沈炳权[2] 金可可[1] 宋张娟[1] 王卫[1] 王青[1] 潘雪蓉[1] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325027 [2]杭州市第四人民医院心内科,浙江杭州310000

出　　处：《中国中西医结合急救杂志》2004年第6期349-351,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：浙江省跨世纪学术和技术带头人基金资助(992 0 86);温州市"551人才工程"培养基金资助(98113);浙江省医药卫生优秀青年科技人才专项基金资助(2 0 0 1QN0 13 );浙江省杭州市医药卫生科技发展计划基金资助(2 0 0 2 A0 99 )

摘　　要：目的 :探讨心肌缺血再灌注损伤 ( MIRI)时乳酸脱氢酶 ( L DH)活性的变化以及中医药的保护作用机制。方法 :实验兔 30只 ,随机分为对照组、盐水组和葛根素葡萄糖注射液 ( PGI)组 ,每组 10只。制作 MIRI模型 ,观察血清及心肌组织 L DH活性、血栓素 B2 ( TXB2 )和 6酮前列腺素 F1α( 6 keto PGF1α)含量、TXB2 /6 keto PGF1α( T/ K)比值变化及 PGI对它们的影响。结果 :MIRI过程中 ,盐水组血清 L DH活性进行性升高( P<0 .0 5或 P<0 .0 1) ,心肌组织内 L DH活性明显下降 ( P<0 .0 1)而 T/ K比值显著增高 ( P<0 .0 1) ;PGI能降低血清中升高的 L DH活性 ,降低心肌组织中 L DH活性和 T/ K比值 ,均有显著性差异 ( P<0 .0 5和 P<0 .0 1)。结论 :PGI可通过纠正 TXA2 与前列环素的平衡 ,对 MIRI时 L DH活性的异常改变起积极的调节作用 。Objective: To explore the changes of lactic dehydrogenase (LDH) activity during myocardial ischemia/reperfusion injury (MIRI) in rabbits, and investigate the protective effect of traditional Chinese medicine on myocardium as well as its mechanisms. Methods: Thirty rabbits were randomly divided into three groups ( n =10 in each group): control group, 0 9% NaCl group, puerarin and glucose injection (PGI) group. Using a MIRI model, the LDH activity, thromboxane B 2(TXB 2) content,6ketoprostaglandin F 1α ( 6keto PGF 1α ) concentration and TXB 2/6ketoPGF 1α (T/K) ratio in serum and myocardium tissue were measured in rabbits. Results: In 0 9% NaCl group, LDH activity increased progressively in serum during MIRI ( P <0 05 or P <0 01), LDH activity in myocardial tissue decreased remarkably( P <0 01), and T/K ratio in myocardial tissue increased significantly( P <0 01). PGI had the effect of decreasing LDH activity in serum that increased during MIRI, decreasing LDH activity and T/K ratio in myocardium, the differences were significant ( P <0 05 and P <0 01). Conclusion: PGI shows a protective effect on myocardium by correcting thromboxane A 2 and prostaglandin I 2 imbalance and modulating the abnormal change of LDH during MIRI.

关 键 词：缺血-再灌注损伤 心肌 乳酸脱氢酶 葛根素葡萄糖注射液 血栓素B1 6-酮-前列腺素F1A 兔 

分 类 号：R285[医药卫生—中药学]