基因物质p63、β1整合素对正常人不同部位皮肤表皮干细胞的表达和意义  

p63 and β1 integrin expression in epidermal stem cells in normal skins at different body parts

作　　者：陈晓东[1] 利天增[1] 祁少海[1] 谢举临[1] 袁即山[1] 徐盈斌[1] 潘姝[1] 张涛[1] 

机构地区：[1]中山大学附属第一医院烧伤科

出　　处：《中华实验外科杂志》2004年第11期1376-1378,共3页Chinese Journal of Experimental Surgery

基　　金：广东省医学科学基金资助项目 (31 685);广东省自然科学基金资助项目 (2 0 0 4 1 85)

摘　　要：目的　探讨基因物质 p63、β1整合素对正常人不同部位皮肤表皮干细胞的表达情况及意义。方法　在 5例正常人尸体上各取 2 1个不同部位的全层皮肤组织 ,采用免疫组织化学SP法 ,用基因物质 p63、β1整合素作为第一抗体标记表皮干细胞 ,并通过图像分析 ,研究表皮干细胞在正常人不同部位皮肤中的表达情况。结果　 (1)头皮 p63的PU值 [阳性单位 (% ) ] (4 2 .17± 3 .10 )最大 ,与面皮、腋部、上臂内侧、前臂内侧、腰背部、手背、足背及足底差异有显著性 (P <0 .0 5 ) ;足背p63的PU值(2 1.19± 6.89)最小 ,与身体其他部位差异均有显著性 (P <0 .0 5 )。头皮CD2 9的PU值 (2 9.13± 4.99)最大 ,与身体其他部位差异均有显著性 (P <0 .0 5 ) ;足底CD2 9的PU值 (11.89±1.5 2 )最小 ,阴囊与面皮、腰背、上臂内外侧、手掌、手背、腹部及足底差异有显著性 (P <0 .0 5 )。(2 )正常人不同部位多毛组与汗毛组 p63、CD2 9的PU值差异有显著性(P <0 .0 0 0 1)。(3 )躯干组与四肢组p63、CD2 9的PU值差异无显著性 (P >0 .0 5 )。(4 )屈侧与伸侧p63、CD2 9的PU值差异无显著性 (P >0 .0 5 )。结论　 (1) p63、CD2 9对正常人不同部位皮肤表皮干细胞的表达不同 ,头皮、阴阜、阴囊皮肤组织中的阳性表达较高 。Objective To investigate the p63 and β1 integrin expression in epidermal stem cells in normal skins at different body parts.Methods Total thickness skin samples were harvested in 5 men,with 21 from different parts each.By the method of SP immunohistochemistry,p63 and β1 integrin was employed as the first antibody to demonstrate the epidermal stem cells.Results (1) The positive unit (PU,%) value of p63 in scalp (42.17± 3.10) was the maximum,statistically different from that in the skins of face,axillary,medial side of arm,medial side of forearm,back,hand,dorsal foot and ventral foot (P< 0.05);The PU value of p63 in dorsal foot (21.19± 6.89) was the minimum (P< 0.05);The PU value of CD29 in scalp (29.13± 4.99)was the maximum,showing statistically significant difference from those from the other sources (P< 0.05);The PU value of CD29 in ventral foot (11.89±1.52) minimum.The PU value of CD29 in scrotum was different from those in face,back,arm,palm,dorsal hand,abdomen,and ventral foot (P< 0.05);(2) The PU values of both p63 and CD29 were different between the skins with thick hair and the others (P< 0.000?1);(3) There were no significant differences in the PU values of p63 and CD29 between the skin of the trunk and those of the extremities (P> 0.05);(4)There was no significant difference in the PU values of p63 and CD29 between the skin of the extension side and that of the flexion side (P> 0.05).Conclusion (1) Both p63 and CD29 expression in epidermal stem cells was different at different body parts.The positive expression in skins of scalp,pubis and scrotum was higher than that in the other parts;(2) As to the identification of epidermal stem cells,the p63 shows higher sensitivity and lower specificity,while the CD29 shows higher specificity and lower sensitivity.

关 键 词：基因物质 P63 Β1整合素 正常人群 皮肤表皮 干细胞 基因表达 CD29 

分 类 号：R346[医药卫生—基础医学]