川芎嗪联用左旋精氨酸抗肝缺血-再灌注损伤作用机制的实验研究  

Mechanism and protective effect of ligustrazini and L-arginine on hepatic ischemia-reperfusion injury in rabbits

作　　者：王万铁[1] 金可可[1] 王卫[1] 陈锡文[2] 徐正衸[1] 许益笑[1] 潘雪蓉[1] 方周溪[3] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325027 [2]温州医学院实验动物中心 [3]温州医学院电镜室

出　　处：《中国急救医学》2004年第12期887-888,共2页Chinese Journal of Critical Care Medicine

基　　金：浙江省跨世纪学术和技术带头人;温州市"5 5 1人才工程"培养基金;浙江省教育厅;卫生厅科研基金 (No 992 0 86;98113 ;95 1118;98A0 87)

摘　　要：目的　探讨川芎嗪 (LGT)联用左旋精氨酸 (L -Arg)对肝缺血 -再灌注损伤 (HIRI)的保护作用及其机制。方法　复制HIRI模型 ,随机将 4 0只实验兔分为肝缺血 -再灌注组 (A组 )、肝缺血 -再灌注 +LGT治疗组 (B组 )、肝缺血 -再灌注+L -Arg治疗组 (C组 )和肝缺血 -再灌注 +LGT +L -Arg治疗组 (D组 )。在再灌注 4 5min时 ,分别观察肝组织内三磷酸腺苷(ATP)、二磷酸腺苷 (ADP)、一磷酸腺苷 (AMP)含量、总腺苷酸量 (TAN)、能荷 (EC)及肝细胞形态学的变化。结果　与A组比较 ,B组、C组、D组肝组织内ATP含量及EC值均明显增高 (ATP :A组为 0 98± 0 2 5 μg/mg,B组为 2 84± 0 77μg/mg,C组为1 88± 0 71μg/mg ,D组为 3 4 9± 0 75 μg/mg ,均P <0 0 1;EC :A组为 0 34± 0 0 5 ,B组为 0 6 1± 0 0 7,P <0 0 1,C组为 0 4 1±0 0 8,P >0 0 5 ,D组为 0 5 7± 0 0 9,P <0 0 1) ;肝细胞形态学异常改变显著减轻 ,D组尤为显著。结论　LGT联用L -Arg可通过改善肝细胞的能量代谢而有效地减轻FIRI。Objective To study the mechanism and protective effect of ligustraxzini(LGT) and L-arginine(L-Arg)on hepatic ischemia-reperfusion injury(HIRI) Methods The HIRI rabbit models were randomly divided into four groups(n=10, in each group),HIR group(A),HIR+LGT group(B), HIR+L-Arg group(C) and HIR+LGT+L-Arg group(D). The contents of adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP),toal adenylic acid number(TAN)and energy charge(EC)in the liver tissue were measured at 4 minutes after reperfusion ,respectively Meanwhile,hepatocellular morphological changes were observed during HIRI Results In B,C,D groups,ATP and EC levels of liver tissue were higher than those in A group(ATP:0 98±0 2 μg/mg,B 2 84±0 77 μg/mg,C 1 88±0 71 μg/mg,D 3 49±0 7 μg/mg,all P <0 01;EC:0 34±0 0 ,B 0 61±0 07, P <0 01,C 0 41±0 08, P >0 0 ,D 0 7±0 09, P <0 01);abnormal morphological changes of liver cells in B,C,D groups were ameliorated remarkably too during HIRI Conclusions Mechanism and protective effect of ligustrazini and L-arginine on hepatic ischemia-reperfusion injury in rabbits$$$$ WANG Wan-tie,JIN Ke-ke,WANG Wei,et al Department of Pathophysiology,Wenzhou Medical College,Wenzhou 32 027,China [Abstract] Objective To study the mechanism and protective effect of ligustraxzini(LGT) and L-arginine(L-Arg)on hepatic ischemia-reperfusion injury(HIRI) Methods The HIRI rabbit models were randomly divided into four groups(n=10, in each group),HIR group(A),HIR+LGT group(B), HIR+L-Arg group(C) and HIR+LGT+L-Arg group(D). The contents of adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP),toal adenylic acid number(TAN)and energy charge(EC)in the liver tissue were measured at 4 minutes after reperfusion ,respectively Meanwhile,hepatocellular morphological changes were observed during HIRI Results In B,C,D groups,ATP and EC levels of liver tissue were higher than those in A group(ATP:0 98±0 2 μg/mg,B 2 84±0

关 键 词：肝脏 缺血-再灌注损伤 能量代谢 川芎嗪 左旋精氨酸 

分 类 号：R285[医药卫生—中药学]