急性CO中毒大鼠脑组织NO NOS活性变化及纳洛酮的干预效应  

Changes in levels of nitric oxide and activities of nitric oxide synthase and intervention of naloxone in rat brain tissue after acute carbon monoxide poisoning

作　　者：王伯良[1] 仲月霞[1] 曹义战[1] 陆将[1] 何保健[1] 江伟[1] 

机构地区：[1]中国人民解放军第四军医大学唐都医院急诊科,陕西西安710038

出　　处：《中国急救医学》2004年第12期889-890,共2页Chinese Journal of Critical Care Medicine

摘　　要：目的　探讨急性一氧化碳 (CO)中毒大鼠脑组织中一氧化氮 (NO)和一氧化氮合成酶 (NOS)活性的变化及纳洛酮的治疗作用。方法　健康Wister大鼠 4 5只 ,随机分为 3组 :正常、CO染毒组 (中毒组 )、CO染毒后纳洛酮治疗组 (观察组 )。采用改良的Griess法和分光度法 ,分别测定大鼠大、小脑组织NO和NOS活性。结果　急性CO中毒后大鼠大、小脑组织中NO和NOS活性明显升高 ,与正常组比较P <0 0 1;应用纳洛酮治疗后 ,脑组织中NO和NOS活性明显降低 ,与中毒组比较P <0 0 1。结论　急性CO中毒后大鼠脑组织中NO和NOS活性增高 ,NO、NOS活性改变可能参与了急性CO中毒脑损伤的病理生理过程 ,纳洛酮治疗可降低急性CO中毒大鼠脑组织中NO和NOS活性。Objective The purpose of this study was to research changes in levels of nitric oxide (NO) and nitric oxide synthase (NOS) and the therapeutical effect of naloxone on rat brain tissue after acute carbon monoxide (CO) poisoning Method Forty-five male Wistar rats were randomly divided into 3 groups:normal control group (normal group);CO exposure (intoxication group);therapeutical group of naloxone after acute carbon monoxide poisoning (observation group) NO,NOS levels were measured by Griess'method,and NOS activity was measured by spectrophotometry Results NO and NOS activity were obviously increased, compared with normal group ( P <0 01);NO and NOS activity of brain were significantly decreased after treating with naloxone,compared with intoxication group ( P <0 01) Conclusion NO and NOS activity of brain tissue were increased after acute carbon monoxide poisoning in rat The changes of NO and NOS activity may take part in pathophysiology process of brain injury of acute carbon monoxide poisoning Treating with naloxone can decrease NO and NOS activity in rat brain tissue of acute CO poisoning [

关 键 词：一氧化碳中毒 一氧化氮 一氧化氮合成酶 纳洛酮 

分 类 号：R595.1[医药卫生—内科学]