汉坦病毒诱导非洲绿猴肾上皮细胞热休克蛋白70的表达规律及意义  

The law of HSP70 expression and significance in Vero-E6 cells induced by hantavirus

作　　者：余璐[1] 马恒[2] 朱军[3] 杨守京[1] 

机构地区：[1]第四军医大学病理学教研室 [2]第四军医大学生理学教研室 [3]第四军医大学西京医院肾内科,陕西西安710032

出　　处：《医学研究生学报》2004年第12期1072-1075,F031,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目 (批准号 :3 9770 664 )

摘　　要：目的 :旨在研究汉坦病毒 (Hantavirus,HTV)感染非洲绿猴肾上皮 (Vero E6 )细胞后应激反应的规律及意义。　方法 :采用免疫细胞化学染色法、免疫荧光激光共聚焦及RT PCR方法观察热体克蛋白 (HSP) 70的表达规律。　结果 :HTV感染Vero E6细胞后 ,免疫细胞化学染色法可检出HSP 70阳性信号。感染后不同时间点 ,免疫荧光激光共聚焦显微镜及RT PCR观察结果表明 ,HSP 70及HSP 70mRNA的水平在感染后迅速升高 ,并持续高表达 (P <0 .0 5 )。　结论 :HTV可诱导Vero E6细胞HSP 70高表达 ;HSPObjective:To study the law and significance of stress response after Vero-E6 cells infected with Hantavirus (HTV). Methods:The law of expression of HSP 70 and its mRNA level were detected by immunocytochemical staining, laser confocal microscope and RT-PCR. Results:After being infected with HTV, immunocytochemical staining exhibited that the expression of HSP 70 increased. laser confocal microscope and RT-PCR detection showed that the level of HSP 70 and its mRNA increased quickly and kept at a high level in comparison with the normal control(P<0.05). Conclusion:The over-expression of HSP 70 in Vero-E6 can be induced by Hantavirus, which may play a role in the inhibition of virus reproduction and the protection of cells from virus infection.

关 键 词：汉坦病毒 热休克蛋白70 应激反应 

分 类 号：R373.32[医药卫生—病原生物学]