血浆蛋白质组学的研究进展  

Progresses of researches on the plasma proteomics

作　　者：郭淑杰[1] 周文霞[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国药理学与毒理学杂志》2004年第6期466-470,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家重点基础研究发展规划基金资助项目(G19990 5 44 0 1) ;国家自然科学基金资助项目 (3 0 2 0 0 3 67)~~

摘　　要：血浆是取材容易、应用广泛的研究样品 ,其蛋白水平变化与多种疾病有关。在所有组织的蛋白质中 ,血浆蛋白质与其mRNA水平相差最远 ,因此血浆是一个只能用蛋白质方法进行研究的生物系统。但是血浆蛋白数量多、来源广泛、蛋白含量差别大 (相差 10个数量级 )等特点限制了血浆蛋白质组研究 ,因此研究者从样品预处理、亚蛋白质组研究和质谱鉴定多个环节尝试突破上述瓶颈 ,进行血浆蛋白质组研究 ,并广泛应用于肿瘤、肾炎等多种疾病的研究中 ,对阐述疾病病理生理学机制、疾病早期诊断、药物效应和不良反应监测具有重要意义。Plasma is a sample to be obtained easily and use d widely for researches. The changes in plasma protein level correlate closely wit h many diseases. Plasma proteome is mostly removed from mRNA level among tissue proteomes, so plasma i s a biological system into which to be given an insight only by protein methods. However, its characteristics of a large amount, multi-source, a wide range of protein concentration (difference in a factor of 10 10 between the highe st abundant protein and the lowest one) limit studies on plasma proteome. Resear chers try to break the bottlenecks mentioned above in sample preparation, subproteomi cs and identification by mass spectrometry in order to clarify plasma proteome. Plasma proteomics is applied to study on tumor, nephritis etc. and w ill con tribute greatly to illuminating pathophysiologic mechanism of diseases, early di agnosis, monitoring of drug effect and toxicity.

关 键 词：血浆 蛋白质组 药理学 

分 类 号：R341[医药卫生—基础医学]