Cathepsin B与扩张型心肌病发病关系的初步探讨  

A Preliminary Study on the Relationship between Cathepsin Band Dilated Cardiomyopathy

作　　者：赵刚[1] 李双杰[2] 陈瑞珍[1] 张新刚[1] 杜九中[2] 李高平[1] 杨英珍[1] 

机构地区：[1]复旦大学附属中山医院-上海市心血管病研究所-卫生部病毒性心脏病重点实验室,上海200032 [2]湖南省南华大学附属第一医院儿内科,湖南衡阳421001

出　　处：《复旦学报（医学版）》2004年第6期556-558,569,F002,共5页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金资助项目 ( 3 0 2 71665 )

摘　　要：目的　探讨溶酶体中CathepsinB与扩张型心肌病发病的关系。 方法　收集我院因终末期扩张型心肌病行心脏移植术的受体标本 2 0例为实验组及脑死亡 3h内排除心脏疾患的心脏标本 5例为正常对照组 ,通过免疫组织化学 ,RT -PCR和WesternBlotting等方法自不同水平对CathepsinB进行检测。 结果　发现两组心肌组织中的CathepsinB均有阳性信号 ;RT PCR半定量检测CathepsinBmRNA的表达示扩张型心肌病组CathepsinB与 β actin条带面积积分比值明显高于对照组 ,分别为 0 .92 6± 0 .30、0 .4 4 2± 0 .2 5 ,两组间有显著差异 (P <0 .0 1) ;同时从蛋白水平检测CathepsinB的表达 ,显示扩张型心肌病组CathepsinB与 β actin条带面积积分比值明显高于正常组 ,分别为 0 .4 5 4± 0 .0 8及 0 .131± 0 .0 3,两组间有显著差异 (P <0 .0 1)。结论　与对照组相比 ,扩张型心肌病心肌标本中CathepsinBmRNA与蛋白质的表达量均明显增高 ,提示CathepsinB可能参与了扩张型心肌病的发生、发展过程 。Purpose: To observe the relationship between lysosomal protease cathepsin B and dilated cardiomyopathy( DCM). Methods: Myocardium tissue specimens derived from 20 patients underwent orthotopic cardiac transplantation and 5 normal myocardium samples were collected autopsy from sudden death as controls. In those myocardium cathepsin B was detected by immunohistochemistry, RT-PCR and Western Blotting, respectively. Results: Immunohistochemistry results showed that the positive signals could be found in the myocardium of DCM, and control group also. However, the expression of cathepsin B mRNA in transplantation myocardium markedly statistically increased, compared with that in control group. Meanwhile, the expression of cathepsin B protein also showed significantly higher in DCM group than that in control group by western blotting. Conclusions: It is presumed that cathepsin B participates in the genesis and development of DCM, and that cathepsin B may play an important role in the myocardial apoptosis in DCM.

关 键 词：扩张型心肌病 对照组 发病关系 正常 表达量 RT-PCR 比值 RNA 标本 溶酶体 

分 类 号：R542.2[医药卫生—心血管疾病] R654.2[医药卫生—内科学]