3株弓形虫GRA7基因的保守性鉴定  

IDENTIFICATION ON CONSERVATION OF GRA7 GENE OF TOXOPLASMA GONDII

作　　者：郑斌[1] 何蔼[1] 李卓雅[1] 申川军[1] 余南[1] 郑焕钦[1] 张瑞琳[1] 李道宁[1] 詹希美[1] 

机构地区：[1]中山大学中山医学院寄生虫学教研室,广东广州510089

出　　处：《中国寄生虫病防治杂志》2004年第5期264-267,共4页Chinese Journal of Parasitic Disease Control

基　　金：国家自然科学基金项目 (No .30 1 70 8377)

摘　　要：目的　比较弓形虫不同地理株 (RH株、ZS2株、GT株 )GRA7基因的异同。　方法　从弓形虫不同地理株的基因组DNA中扩增出GRA7基因 ,对目的基因用限制性内切酶 (Esp3I、CfrI、MboI)酶切并比较。将目的基因克隆至pGEX 4T 1质粒 ,转化大肠埃希菌JM 10 9,进行测序并比较。　 结果　PCR扩增出 3株弓形虫的目的基因片段 ,大小均在 5 0 0～ 75 0bp之间 ;经 3种限制性内切酶酶切 ,其大小均与理论值相符 ;3株弓形虫GRA7基因序列相同。　Objective To compare the GRA7 gene from different geographical Toxoplasma gondii strains(RH, ZS 2, GT). Methods PCR technique was used to amplify GRA7 gene from genomic DNA of different geographical T. gondii strains, the GRA7 gene were digested by the restriction endonuclease (Esp3I, CfrI, MboI) and cloned into plasmid pGEX-4T-1.The reconstructed plasmids were transfered into Escherichia coli JM109 and sequenced. Results The GRA7 gene was successfully amplified from the genomic DNA of different geographical T. gondii strains. The digested fragments by restriction endonuclease were not significantly different. The sequences of GRA7 gene from different geographical T. gondii strains were same. Conclusion The GRA7 gene from different geographical T. gondii strains (RH, ZS 2, GT) has high conservation.

关 键 词：弓形虫 GRA7基因 限制性内切酶 克隆 

分 类 号：R382.5[医药卫生—医学寄生虫学]