未成熟树突状细胞诱导协调性异种胰岛移植耐受的研究  

Study on the immune tolerance induced by immature dendritic cells in coordination islet xenotransplantation

作　　者：赵刚[1] 王芳[2] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430022 [2]华中科技大学同济医学院药理系

出　　处：《中华器官移植杂志》2004年第6期371-373,共3页Chinese Journal of Organ Transplantation

摘　　要：目的　研究未成熟树突状细胞 (DC)在协调性异种胰岛移植中的免疫耐受诱导作用。方法　分别应用 2 0 μg/L粒细胞集落刺激因子 (GM CSF)和 5 0 0 μg/LGM CSF +2 0 0 μg/L白细胞介素4 (IL 4) ,从BALB/c受鼠骨髓培养未成熟DC及成熟DC ,然后负载Wistar大鼠的主要组织相容性复合物 (MHC)抗原。将两种DC回输至糖尿病小鼠体内 ,7d后分别将Wistar大鼠和SD大鼠胰岛移植于糖尿病小鼠左肾包膜下 ,监测移植物存活情况 ,检测T细胞增殖反应 ,并进行病理切片检查。结果　负载MHC抗原成熟DC预处理的BALB/c小鼠体内胰岛迅速被排斥 ,负载MHC抗原未成熟DC预处理的BALB/c小鼠胰岛存活时间明显延长 (P <0 .0 1 ) ,但以SD大鼠作为供者的胰岛移植物迅速被排斥。耐受鼠的脾细胞对Wistar大鼠脾细胞的增殖反应微弱 ,而排斥鼠脾细胞则出现明显增殖反应。结论　未成熟DC预处理受者可诱导T细胞无能 ,并有效延长异种胰岛移植后的存活时间。Objective To investigate the inducible effect of immature dendritic cells (DC) on immune tolerance in coordination islet xenotransplantation.Methods Mature and immature DC were cultured from BALB/c mice with 20 μg/L GM-CSF and 500 μg/L GM-CSF+ 200 μg/L IL-4, respectively, then loaded with major histocompatibility complex (MHC) antigen of Wistar rat. Mature and immature DC were injected into the mice with diabetes. After a week, the islets of Wistar rat or SD rat were transplanted under the envelop of left renal of the diabetes mice. The survival time of grafts was monitored. The immune reactivity of T cell was evaluated by mixed lymphocyte culture. Results Grafts in BALB/c mice pretreated with mature DC rejected quickly, while the survival time of grafts was significantly prolonged in the mice pretreated with immature DC. However, whether pretreated with mature DC or immature DC, grafts from SD rat were rejected soon. The splenocytes of tolerant mice showed mild proliferation. Conclusion The recipient pretreated with immature DC will lead to the anergy of T lymphocyte, and prolong the survival time of xeno-islet.

关 键 词：异种胰岛移植 未成熟树突状细胞 DC Wistar大鼠 耐受 抗原 MHC 协调性 结论 受者 

分 类 号：R657.5[医药卫生—外科学]