华支睾吸虫可溶性抗原对BALB/C鼠免疫效果的研究  

Study on Immune Effect of BALB/C Mice with Soluble Antigen of Clonorchis Sinensis

作　　者：潘赛贻[1] 周洁娴[1] 黄健康[1] 

机构地区：[1]广东省寄生虫病防治研究所,510300

出　　处：《广东卫生防疫》1998年第3期1-3,共3页Guangdong Journal of Health and Epidemic Prevention

基　　金：广东省医药卫生科研基金

摘　　要：用华支睾吸虫成虫可溶性抗原加等量自制佛氏完全佐剂（FCA）免疫BAU3／C鼠，以不同免疫途径、不同时间观察其免疫效果。结果腹腔注射组和皮下多点注射组两次基础免疫间隔3周，第二次基免后两周抗体几何平均滴度分别为2560．00和1470．00，差异无显著性；以加倍剂量于基础免疫后19－122天进行加强免疫，抗体水平迅速提高，于第三天已超过基础免疫水平，但在此时间内进行加强免疫的效果差异无显著性；两次基础免疫间隔3周和4周，其加强免疫后的抗体几何平均滴度分别为5881.34和3044.37，前者优于后者。实验中分别用自制FCA和进口FCA加入可溶性抗原进行免疫，其效果基本一样，但前者制作简便，价廉实用，可取代进口FCA。BALB/C face were immunized with the mixture of soluble antigen of Clonorchis sinensis adult worms and equivalent Freund' s complete adjuvant(FCA), and the immune effects of different routes and time intervals were observed. The results showed that the geoetric mean titer of serum antibody of intraperitaneal and subcutancous primary immunization (two-dose with 3 weeks internal) were 2500. 00 and 1470. 00 respectively, two weeks after the second dose. The difference had no significance. The antibody levels of booster immunization given 19 -122 days after the primary increased rapidly and went beyond the level of primary immunization at the third day. The titers of 3 -week and 4 -week interval were 5881 .34 and 3044. 37 respectively. In addition, the immune effects of self-made FCA and imported rcA were basically the same. The former, easy to make, cheap and practical, can substitute imported FCA.

关 键 词：基础免疫 加强免疫 华支睾吸虫 可溶性抗原 抗体几何平均滴度 BALB/C 免疫效果 加倍 等量 显著性 

分 类 号：R186[医药卫生—流行病学] R512[医药卫生—公共卫生与预防医学]