大鼠胰腺不同发育时期转录因子Nkx2.2和Nkx6.1mRNA的表达调控研究  

Research of the homeodomain transcription factors Nkx2.2 and Nkx6.1 expression at different development periods in rat pancreas

作　　者：张春举[1] 袁栎[1] 王林涛[1] 周锦勇[1] 钟燕[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学和分子生物学系

出　　处：《南京医科大学学报（自然科学版）》2005年第1期17-19,共3页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:研究大鼠胚胎胰腺发育后期、新生期及成年期对转录因子Nkx2.2和Nkx6.1的mRNA表达调控。方法:利用显微分离等方法分离胚胎18.5天、新生和成年大鼠胰腺,对胰腺标本提取总RNA,利用胰腺内外分泌细胞分子标志———胰岛素和淀粉酶来鉴定胰腺标本,再用RT鄄PCR分析Nkx2.2或Nkx6.1在大鼠胰腺几个不同发育时期的mRNA表达情况。结果:Nkx6.1在胚胎18.5天胰腺中有表达,出生时表达量有增加,到了成年显著下降。Nkx2.2的mRNA在胚胎期、新生期和成年期均有表达,新生期表达量较其他两个时期有显著性增加。从以上两个转录因子在胰腺不同发育时期的表达情况来看,它们的共同点是胚胎期有表达,新生期表达量较胚胎后期和成年期高,成年期表达量呈下降趋势。结论:不同生长发育时期对转录因子Nkx2.2和Nkx6.1的mRNA具有调节作用,新生期细胞增生分化加速,这与Nkx2.2、Nkx6.1等转录因子表达量增高有关。Objective: To study the effect of the different development periods to the homeodomain transcription factors Nkx2.2 and Nkx6.1 expression in rat pancreas. Methods: Pancreas were isolated from SD rats on gestational days 18.5, new born and adult by microscope. Pancreas were probed by the pancreatic molecule marker-insulin and amylase. A semi-quantitative reverse transcriptase polymerase chain reaction(RT-PCR) was performed to assess the expression of transcription factors Nkx2.2 and Nkx6.1 in different developing periods in rat pancreas. Results: Nkx2.2 and Nkx6.1 gene expression increased from gestation to new born and then decreased in adult. Conclusion: Different development periods can regulate the expression of Nkx2.2 and Nkx6.1 gene. New born period is a special time when loss nutrition from mother body, and initial of autoimmune and digestion system. The resire in cell neogenesis may lead to the increase in the gene expression of the homeodomain transcription factors Nkx2.2 and Nkx6.1.

关 键 词：Nkx2.2 Nkx6.1 Β细胞 转录因子 胚胎胰腺 

分 类 号：R333.3[医药卫生—人体生理学] R335.6[医药卫生—基础医学]