人胎盘泌乳素化学发光免疫分析方法的建立  

The Establishment of a Chemiluminescence Immunoassay for Human Placental Lactogen

作　　者：叶成果 屈凌波[2] 李建军[2] 王晓谦 刘均生 

机构地区：[1]华美生物工程公司,河南洛阳471003 [2]郑州大学化学系,河南郑州450052

出　　处：《河南科技大学学报（医学版）》2004年第4期245-246,共2页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的　建立人血清胎盘泌乳素 (HPL)的化学发光免疫分析方法。方法　以HPL单克隆抗体作为固相包被 ,HPL多克隆抗体与辣根过氧化物酶偶联制备酶标抗体 ,以鲁米诺作为底物 ,采用两步法建立了双抗体夹心法人血清HPL的化学发光免疫定量分析法。结果　该方法的敏感度为 0 .1mg/L ,可测范围为 0 .1～ 2 0mg/L ,批内和批间的变异率分别为 3 .82 %和 7.43 % ,回收率在 85 %～ 1 1 5 %之间。本法与HGH的交叉为 0 .0 45 % ,与垂体泌乳素的交叉为 0 .0 76% ,与HCG的交叉为 0 .0 0 3 2 % ,血清样品测定结果与进口HPL放射免疫检测试剂盒测定结果的相关系数为 0 .983。结论　建立的方法用于HPL的检测快速、敏感、准确 。Objective To develop an enzyme-linked chemiluminescence immunosorbent assay for human placental lactogen(HPL)in human serum. Methods The two-site assay was based on the direct sandwich technique. A monoclonal antibody was employed for coating, another polyclonal antibody for labeling with horseradish peroxidase. Luminol was used for the substrate. Result The minimum detectable dose was 0 1mg/L. The linear response was from 0.1 to 20mg/ml.The between-runs CVs and the within-runs CVs were 7 43% and 3 82%, respectively. The analytical recovery was 85% to 115%. The cross-reacting rate with HGH was 0 045%,and that with prolactin and HCG was 0 076% and 0 0032%, respectively. Compared with the imported RIA kit,the correlation coefficient was 0 983. Conclusion The method provides a rapid,sensitive and exact determination of HPL and is suitable for application to clinic diagnosis

关 键 词：人胎盘泌乳素 化学发光免疫分析法 鲁米诺 碘苯酚 酶联免疫法 

分 类 号：R392-33[医药卫生—免疫学]