超氧化物歧化酶模型化合物在金电极上的分子自组装  

Self assembly of a model compound of superoxide dismutase on gold electrode

作　　者：陈涛[1] 张爱东[1] 潘克华[1] 梅伏生[1] 廖展如[1] 

机构地区：[1]华中师范大学化学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2004年第4期455-458,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金资助项目(20241001);湖北省自然科学基金资助项目(2002AB055).

摘　　要：采用3-巯基丙酸在金电极上自组装得到自组装单分子层(SAM),再利用DCC作酰胺键偶合剂,将超氧化物歧化酶(SOD)模型化合物Fe(IDB)Cl3(简称Fe-MSOD,IDB为N,N-二(2′-苯并咪唑甲基)亚胺)共价键合到自组装在金电极表面的3-巯基丙酸单分子层上,构筑了一种超氧化物歧化酶模型化合物分子组装体系膜修饰电极(Fe-MSOD/3-MPA/Au).采用电化学循环伏安法、交流阻抗法对该修饰电极进行了表征.初步探讨了此修饰电极的电化学氧化还原性质.3-Mercaptopropane acid (3-MPA) was first used to fabricate the self-assmebled monolayer(SAM) on gold electrode, then dicyclohexylcarbodiimide (DCC) as coupling reagent was used to form amide linkage between the terminal carboxylic group of the self-assembled monolayer and the amino group of a model compound of superoxide dismutase Fe(IDB)Cl_3 (briefly Fe-MSOD, IDB: N,N-bis((benzimidol-2-yl)methyl)amine). The electrochemical cyclic voltammetry and impedance were employed to characterize the formation of molecular assembly system on gold, and the results show that the model compound had been fixed on the surface of the monolayer with a surface coverage of 5.67×10^(-11) mol/cm^2. The Fe-MSOD on the monolayer presented a quasi-reversible electrochemical redox.

关 键 词：超氧化物歧化酶 模型化合物 修饰电极 交流阻抗 循环伏安 

分 类 号：O646.22[理学—物理化学]