几种营养因子对半夏悬浮细胞生长及总生物碱形成的影响  

Effects of nutritional factors on cell growth and total alkaloids formation in suspension cultures of Pinellia ternata(Thunb.)Breit

作　　者：范美华[1] 冯玲玲[1] 彭瑜[1] 杨春燕[1] 张泽宏[1] 周吉源[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2004年第4期493-496,共4页Journal of Central China Normal University：Natural Sciences

摘　　要：研究了五种营养因子对半夏悬浮细胞生长及总生物碱形成的影响.结果表明,2%～5%的葡萄糖是最为适合的碳源;肌醇对细胞生长的促进效果不大,但100mg/L的肌醇对总生物碱的积累效果显著;钙盐和EDTA-Fe盐对细胞生长和总生物碱形成的影响比较显著,其最适浓度分别为1.8～3.0mmol/L和0.06mmol/L.Studies on the effect of nutritional factors on the cell growth and alkaloids formation in suspension culture of Pinellia ternata(Thunb.)Breit was carried out. The results showed that glucose was better than sucrose for cell growth and alkaloids synthesis and the optimum concentration was 2%~5%. Although inositol had little effect on the cell growth, 100 mg/L inositol was best for alkaloids synthesis. Calcium and EDTA-Fe had obvious effects on the cell growth and alkaloids production and the optimum concentrations were 1.8~3.0 mmol/L and 0.06 mmol/L respectively.

关 键 词：半夏 悬浮培养 总生物碱 营养因子 细胞生长 

分 类 号：Q942[生物学—植物学]