南黄海潮汐锋对浮游细菌生物量分布的影响  

Impact of the tidal front on the distribution of bacterioplankton biomass in the southern Yellow Sea

作　　者：李洪波[1] 肖天[1] 刘桂梅[1] 吕瑞华[2] 丁涛[3] 

机构地区：[1]中国科学院海洋研究所海洋生态与环境重点实验室 [2]国家海洋局第一海洋研究所,青岛266000 [3]国家海洋局海洋动力过程与卫星海洋学重点实验室

出　　处：《生态学报》2004年第11期2608-2615,共8页Acta Ecologica Sinica

基　　金：国家重点基础研究专项经费资助项目 ( G19990 43 7) ;国家自然基金资助项目 ( 4 0 3 760 48)~~

摘　　要：2 0 0 1年 5月 16～ 2 3日、6月 10～ 2 4日和 2 0 0 2年 6月 5～ 12日 ,利用“北斗号”船只对南黄海鱼产卵场进行了 3次专项调查。研究了潮汐锋断面叶绿素 a浓度、浮游细菌生物量的分布 ,目的是阐明潮汐锋的存在对浮游细菌生物量分布的影响。3个航次中的叶绿素 a浓度变化范围分别是 0 .0 6～ 2 .34mg/ m3(2 0 0 1- 0 5 )、0 .0 8～ 0 .9mg/ m3(2 0 0 1- 0 6 )、0 .14～ 3.0 4 mg/ m3(2 0 0 2 - 0 6 )。 3航次的聚球藻 (Synechococcus spp.)蓝细菌生物量变化范围分别为 7.6 2～ 2 2 .0 6 mg C/ m3(2 0 0 1- 0 5 )、8.5 3～2 7.5 2 mg C/ m3(2 0 0 1- 0 6 )、0 .6 9～ 5 5 .90 m g C/ m3(2 0 0 1- 0 6 )。异养细菌生物量变化范围分别为 7.5 6～ 5 1.82 mg C/ m3(2 0 0 1-0 5 )、8.5 4～ 2 4 .77mg C/ m3(2 0 0 1- 0 6 )、3.12～ 10 .0 5 mg C/ m3(2 0 0 2 - 0 6 )。而聚球藻蓝细菌对浮游植物总生物量的贡献 (CB:PB)平均值分别为 :5 8% (2 0 0 1- 0 5 )、77% (2 0 0 1- 0 6 )、31% (2 0 0 2 - 0 6 )。结果表明 :南黄海鱼产卵场在春末夏初 (5～ 6月份 ) ,叶绿素 a浓度最大值及次大值主要分布在锋区及其邻近的层化区2 0 m以浅位置 ;聚球藻蓝细菌生物量最大值主要分布于锋区及层化区表层和水体中层 ;Bacterioplankton is composed mainly of heterotrophic bacteria and two types of photosynthetic prokaryote cyanobacteria, Prochlorococcus and Synechococcus. They are basic components of microbial loop, and play an important role in the marine food web. In spring and summer, the tidal front, a significant hydrological feature of the Yellow Sea, plays a key role in the distribution of zooplankton and phytoplankton. To study the influence of tidal front on bacterioplankton, three special surveys were carried out in May and June, 2001, and June, 2002 in the southern Yellow Sea, China. Chlorophyll a (Chl-a) concentration, bacterioplankton abundance and biomass were quantified and the variation of these three parameters within the zone of tidal front was compared. Samples for bacterioplankton were collected from a SeaBird CTD-General Oceanic Rosette assembly with 10 l Go-Flo bottles, and preserved with buffered glutaraldehyde at a concentration of 1%. Concentrations of Chl-a were measured on a Turner fluorometer. Phytoplankton biomass was calculated by a conversion factor of 50 mg C/mg chlorophyll. Synechococcus cyanobacteria abundance was determined by directly counting cell numbers using epifluorescence microscopy method; and heterotrophic bacteria after acridine organge staining. The biomass of Synechococcus cyanobacteria and heterotrophic bacteria were calculated with a carbon conversion factor of 294 fg/cell and 20 fg/cell, respectively. The Chl-a concentration ranged from 0.06 to 2.34 mg C/m^(3) in May, 2001, from 0.08 to 0.9mg C/m^(3) in June, 2001 and from 0.14 to 3.04mg C/m^3 in June, 2002. The maximum Chl-a concentration was observed in the frontal zone and the neighboring stratified zone. The biomass and abundance of Synechococcus ranged from, respectively, 7.62 to 22.06mg C/m^3 and 2.59 to 7.5×10~4 cells/ml in May, 2001, from 8.53 to 27.52 mg C/m^3 and 2.90 to 27.52×10~4 cells/ml in June 2001, and 0.69 to 55.90 mg C/m^3 and 0.23 to 19.01×10~4 cells/ml in June, 2002. Synechococcus was found mainly in the

关 键 词：潮汐锋 浮游细菌 生物量 黄海 

分 类 号：Q938[生物学—微生物学]