A candidate DNA vaccine elicits HCV specific humoral and cellular immune responses  

作　　者：Li-XinZhu JingLiu YeYe You-HuaXie Yu-YingKong Guang-DiLi YuanWang 

机构地区：[1]StateKeyLaboratoryofMolecularBiology,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,Shanghai200031,China

出　　处：《World Journal of Gastroenterology》2004年第17期2488-2492,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the National High Technology R&D Program of China,No.2001AA215171

摘　　要：AIM: TO investigate the immunogenicity of candidate DNA vaccine against hepatitis C virus (HCV) delivered by two plasmids expressing HCV envelope protein 1 (El) and envelope protein 2 (E2) antigens respectively and to study the effect of CpG adjuvant on this candidate vaccine.METHODS: Recombinant plasmJds expressing HCV EI and E2 antigens respectively were used to simultaneously inoculate mice with or without CpG adjuvant. Antisera were then collected and tJters of antJ-HCV antibodies were analyzed by ELISA. One month after the last injection, animals were sacrificed to prepare single-cell suspension of splenocytes.These cells were subjected to HCV antigen specific proliferaion assays and cytokine secretion assays to evaluate the cellular immune responses of the vaccinated animals.RESULTS: Antibody responses to HCV EI and E2 antigens were detected in vaccinated animals. Animals receiving CpG adjuvant had slightly lower titers of anti-HCV antibodies in the sera, while the splenocytes from these animals showed higher HCV-antigen specific proliferation. Analysis of cytokine secretion from the splenocytes was consistent with the above results. While no antigen-specific IL-4 secretion was detected for all vaccinated animals, HCV antigen-specific INF-γ, secretion was detected for the splenocytes of vaccinated animals. CpG adjuvant enhanced the secretion of INF-γ, but did not change the profile of IL-4 secretion.CONCLUSION: Vaccination of mice with plasmids encoding HCV E1 and E2 antigens induces humoral and cellular immune responses. CpG adjuvant significantly enhances the cellular immune response.AIM:To investigate the immunogenicity of candidate DNA vaccine against hepatitis C virus(HCV)delivered by two plasmids expressing HCV envelope protein 1(E1)and envelope protein 2(E2)antigens respectively and to study the effect of CpG adjuvant on this candidate vaccine. METHODS:Recombinant plasmids expressing HCV E1 and E2 antigens respectively were used to simultaneously inoculate mice with or without CpG adjuvant.Antisera were then collected and liters of anti-HCV antibodies were analyzed by ELISA.One month after the last injection,animals were sacrificed to prepare single-cell suspension of splenocytes. These cells were subjected to HCV antigen spedfic proliferalion assays and cytokine secretion assays to evaluate the cellular immune responses of the vaccinated animals. RESULTS:Antibody responses to HCV E1 and E2 antigens were detected in vaccinated animals.Animals receiving CpG adjuvant had slightly lower titers of anti-HCV antibodies in the sera,while the splenocytes from these animals showed higher HCV-antigen specific proliferation.Analysis of cytokine secretion from the splenocytes was consistent with the above results.While no antigen-specific IL-4 secretion was detected for all vaccinated animals,HCV antigen-specific INF-γ secretion was detected for the splenocytes of vaccinated animals.CpG adjuvant enhanced the secretion of INF-γ but did not change the profile of IL-4 secretion. CONCLUSION:Vaccination of mice with plasmids encoding HCV E1 and E2 antigens induces humoral and cellular immune responses.CpG adjuvant significantly enhances the cellular immune response.

关 键 词：DNA疫苗 HCV 特效药 体液 细胞免疫反应 抗体 

分 类 号：R512.63[医药卫生—内科学]