Changes of mRNA expression of enkephalin and prodynorphin in hippocampus of rats with chronic immobilization stress  

作　　者：Jia-XuChen WeiLi XinZhao Jian-XinYang Hong-YanXu Zhu-FengWang Guang-XinYue 

机构地区：[1]SchoolofBasicMedicalScience,BeijingUniversityofTraditionalChineseMedicine,Beijing100029,China

出　　处：《World Journal of Gastroenterology》2004年第17期2547-2549,共3页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.30000216,Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutes of Ministry of Education,China,Foundation for the Authors of National Excelle

摘　　要：AIM: To observe the changes of enkephalin mRNA and prodynorphin mRNA in hippocampus of rats induced by chronic immobilization stress.METHODS: Thirty rats were randomly divided into three groups of 10 each: the normal control group (group A),the group induced by chronic immobilization stress for 7 d (group B) and the group induced by chronic immobilization stress for 21 d (group C). The changes of the enkephalin mRNA and prodynorphin mRNA in the rat hippocampus were detected by reverse transcription-polymerase chain reaction (RT-PCR).RESULTS: Expression levels of enkephalin mRNA and prodynorphin mRNA in rat hippocampus were significantly increased under chronic immobilization stress, and the expression of prodynorphin mRNA in the rat hippocampus in group C was remarkably higher than that in group B (0.624±0.026; n = 5; P<0.01).CONCLUSION: The increased enkephalin mRNA and prodynorphin mRNA gene expressions in rat hippocampus were involved in chronic stress.AIM:To observe the changes of enkephalin mRNA and prodynorphin mRNA in hippocampus of rats induced by chronic immobilization stress. METHODS:Thirty rats were randomly divided into three groups of 10 each:the normal control group(group A), the group induced by chronic immobilization stress for 7 d (group B)and the group induced by chronic immobilization stress for 21 d(group C).The changes of the enkephalin mRNA and prodynorphin mRNA in the rat hippocampus were detected by reverse transcription-polymerase chain reaction(RT-PCR). RESULTS:Expression levels of enkephalin mRNA and prodynorphin mRNA in rat hippocampus were significantly increased under chronic immobilization stress,and the expression of proclynorphin mRNA in the rat hippocampus in group C was remarkably higher than that in group B (0.624+0.026;n=5;P<0.01). CONCLUSION:The increased enkephalin mRNA and prodynorphin mRNA gene expressions in rat hippocampus were involved in chronic stress,

关 键 词：mRNA表达 脑啡肽 海马神经 老鼠 慢性压力作用 基因表达 正强啡肽 

分 类 号：R318[医药卫生—生物医学工程]