海水浸泡对保存血液中2,3-DPG、ATP含量与pH值影响的实验研究  

The studies on changes of 2,3-DPG,ATP and pH in the bagged blood dipped in seawater

作　　者：冯国基 郑长青 刘景汉[2] 韩玮[2] 朱秀美 施庆忠 

机构地区：[1]401医院,山东青岛266071 [2]解放军总医院,北京100080

出　　处：《实用医药杂志》2004年第10期910-913,共4页Practical Journal of Medicine & Pharmacy

基　　金：十五全军指令性课题资助项目(01L045)

摘　　要：目的观察经海水浸泡的血袋血液2,3-DPG、ATP与pH指标的变化规律,探索海洋环境下血液的运输和保存。方法三种血液制品分为浸泡实验组与对照组,选取海水浸泡前、浸泡72h后、再置4±2℃冰箱保存1、2、3、4、5周各时间点测定成分血液2,3-DPG、ATP的含量与pH值。结果①实验组与对照组比较:2,3-DPGgnATP含量和pH值三种血液制品均有显著性差异(P<0.05或P<0.01);②保存时间内各组比较:2,3-DPG含量在LP-RBC浸泡前、浸泡72h和保存1周与保存2～5周P<0.01;AS-RBC浸泡前、浸泡72h和保存1～2周与保存3～5周P<0.01;WB浸泡前、浸泡72h和保存1～3周与保存4～5周P<0.01;ATP含量LP-RBC与AS-RBC各组比较,P<0.05;WB浸泡前、浸泡72h和保存1～2周与保存3～5周比较,P<0.01;pH值在三种血液制品各组两两比较均有统计学差异(P<0.01);③三种血液制品比较:2,3-DPG含量在LP-RBC、AS-RBC与WB实验组P<0.01,ATP含量在三种血液制品组间比较,P>0.05;pH值除LP-RBC与AS-RBC实验组在保存第5周组间无差别外,其余各血液制品间各实验组比较均有显著性差异(P<0.01)。结论采用相同的血液保养液袋,对血液再包装进行海水浸泡72h,与常规条件下保存血液的2,3-DPG、ATP含量、pH值等指标结果变化趋于一致。Objective To observe the change rule of 2,3-DPG, ATP and pH in the bagged blood dipped in seawater.Method Three kind of blood products were even divided into two groups marking dipped in seawater group and control group. 2,3-DPG, ATP and pH in these blood products were detected respectively in different time before dipped in seawater ,three days after dipped in seawater till five weeks storing in 4±2℃ refrigerator. Results In all three blood products, the change of 2,3-DPG was remarkable different after three days dipped in seawater (P<0.01); but there was no notable different between the groups of experiment and control (P>0.05). The changes of ATP and pH were remarkable different between before dipped in seawater and after dipped in seawater for 72hours and between the groups of the experiment and control (P<0.01). Conclusion The rule of changes of 2,3-DPG, ATP and pH in the blood products are inclined to the coherence when they are stored in uniform blood preser-vation bag regularly and dipped in seawater for 72hours, so the blood after dipped in seawater 72hours can be used in clinic.

关 键 词：海水浸泡 保存血液 2 3—二磷酸甘油酸 三磷酸腺苷pH值 

分 类 号：R832[医药卫生—航空、航天与航海医学] R84[医药卫生—临床医学]