Spectroscopy Investigation on Conformational Transition of Tea Glycoconjugate from Green Tea  

作　　者：陈海霞 张民 谢笔钧 

机构地区：[1]Food Science and Technology Department, Huazhong Agricultural University Wuhan, Hubei 430070, China [2]Institute of Marine Drug and Food, Ocean University of China, Qingdao, Shandong 266003, China

出　　处：《Chinese Journal of Chemistry》2004年第11期1387-1390,共4页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (No. 30270939).

摘　　要：The conformational transition of a new glycoconjugate, tea glycoconjugate (TGC), was investigated by spec-troscopy techniques including circular dichroism (CD) and ultraviolet (UV) spectroscopy. The solution behaviors of TGC in the mediums of different temperature, pH value, and ions were compared. Results showed that the native conformation of TGC was partially ordered. The CD value and UV absorbance of TGC altered with the change of pH value, temperature, the addition of ions, and also accompanied order-disorder transition. Especially the condi-tions with temperature higher than the glass transition temperature (Tg=62 ℃), higher pH value or lower pH value will have the most impact on the conformation of TGC, which will destroy the hydrogen bonds between the TGC molecules. The results indicated that the outside factors play important roles on the stability of the conformation of TGC.The conformational transition of a new glycoconjugate, tea glycoconjugate (TGC), was investigated by spec-troscopy techniques including circular dichroism (CD) and ultraviolet (UV) spectroscopy. The solution behaviors of TGC in the mediums of different temperature, pH value, and ions were compared. Results showed that the native conformation of TGC was partially ordered. The CD value and UV absorbance of TGC altered with the change of pH value, temperature, the addition of ions, and also accompanied order-disorder transition. Especially the condi-tions with temperature higher than the glass transition temperature (Tg=62 ℃), higher pH value or lower pH value will have the most impact on the conformation of TGC, which will destroy the hydrogen bonds between the TGC molecules. The results indicated that the outside factors play important roles on the stability of the conformation of TGC.

关 键 词：tea glycoconjugate conformational transition circular dichroism ultraviolet spectroscopy 

分 类 号：O657.3[理学—分析化学]