超灵敏葡萄糖生物传感基于自组装纳米金和(3-巯基丙基)-三甲氧基硅烷凝胶溶胶(英文)  

Glucose Based on Self-Assembling Gold Nanoparticles and MPS Sol-Gel

作　　者：钟霞[1] 袁若[1] 柴雅琴[1] 赵勤[2] 刘颜[1] 唐点平[1] 戴建远[1] 

机构地区：[1]西南师范大学化学化工学院,重庆市现代分析化学重点实验室,重庆400715 [2]南通工学院化学工程系,江苏南通226007

出　　处：《西南师范大学学报（自然科学版）》2004年第6期976-980,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(29705001);教育部优秀青年教师资助计划(教人司2002-40);重庆市自然科学基金资项目(2002-7477).

摘　　要：采用一种新颖的方法将(3 巯基丙基) 三甲氧基硅烷凝胶溶胶、纳米金和葡萄糖氧化酶自组装于金电极表面,制得了 高灵敏度的葡萄糖生物传感器.实验表明固定在三维网状的凝胶溶胶中的葡萄糖氧化酶和纳米金颗粒具有很强的稳定性并 且能够保持自身的活性.本实验采用Co(bpy)3+3作为电子媒介体,峰电流值与葡萄糖浓度在8.0×10-10～4.2×10-8mol/L 成线形关系,检测下线为3.0×10-10mol/L(S/N=3).A novel method for glucose biosensor based on self-assembling monolayers(SAMs) formation of glucose oxidase(GOD)-gold nanoparticles-(3-mercaptopropyl)-trimethoxysilane(MPS) sol-gel on gold substrate is described in this work. The GOD and gold nanoparticles immobilized on MPS gel were stable and retained its functional activity. With using Co(bpy)^(3+)_3 as a mediator, the peak currents were proportional to the glucose concentration from 8.0×10^(-10) to 4.2×10^(-8) mol/L and the detection limit was as low as 3.0×10^(-10) mol/L(S/N=3) toward glucose.

关 键 词：自组装 胶溶 三甲氧基硅烷 溶胶 纳米金 网状 金电极 巯基 凝胶 灵敏 

分 类 号：O648[理学—物理化学] R587[理学—化学]