抗汉滩病毒单抗3G1 scFv植物表达载体的构建  

Construction of Plant Expression Vector scFv Gene Fragment of mAb 3G1 Against HTNV

作　　者：罗雯[1] 张九东[1] 白文涛[2] 吴兴安[2] 胡刚[2] 张芳琳[2] 

机构地区：[1]西安文理学院生命科学系,陕西西安710065 [2]第四军医大学微生物学教研室,陕西西安710032

出　　处：《西安联合大学学报》2004年第5期5-7,共3页

基　　金：国家自然科学基金资助(项目编号:30471626);西安市科技局社会与发展计划资助(项目编号:200345)

摘　　要：利用PCR方法,从含有3G1scFv基因的重组质粒中扩增出抗体基因,并使基因两端携带合适的限制性酶切位点.将其克隆入植物表达载体pBI121,构建获得3G1scFv pBI121重组质粒.酶切鉴定及测序结果均证明重组质粒构建成功.将重组植物表达载体转入农杆菌LBA4404,为进一步构建转基因植物的研究奠定了基础.3Gl scFv gene fragment was amplified and proper restriction enzyme sites were added at each end by PCR. It was cloned into pBI121 to make recombinant 3Gl scFv-pBI121, which was proved to be constructed successfully by both restriction enzyme analysis and sequencing. The recombinant plant expression vector was further transferred to LBA4404. This has laid a solid foundation for further study on construction of transgenic plant.

关 键 词：汉滩病毒 单链抗体 植物表达载体 

分 类 号：Q78[生物学—分子生物学]