Effect of Cerium on Activity of α-Amylase from Porcine Pancreas  

作　　者：王雪峰 洪法水 沈颂东 苏国兴 潘兴法 

出　　处：《Journal of Rare Earths》2002年第5期553-556,共4页稀土学报（英文版）

基　　金：ProjectsupportedbytheResearchFoundationofSuzhouUniversity"2 1 1Project" (XQ3 1 6 0 1 1 )

摘　　要：The activity of α amylase from porcine pancreas was enhanced under the treatment by Ce 3+ of low concentration (0.5～10 μmol·L -1 ), but was inhibited by Ce 3+ of high concentration (>10 μmol·L -1 ). Ce 3+ at high concentration displaced Ca 2+ from α amylase competitively. The equilibrium dialysis demonstrates that α amylase has five Ca 2+ binding sites with different affinities. The fluorescence titration shows that Ce 3+ can bind to Ca 2+ binding sites.The activity of α amylase from porcine pancreas was enhanced under the treatment by Ce 3+ of low concentration (0.5～10 μmol·L -1 ), but was inhibited by Ce 3+ of high concentration (>10 μmol·L -1 ). Ce 3+ at high concentration displaced Ca 2+ from α amylase competitively. The equilibrium dialysis demonstrates that α amylase has five Ca 2+ binding sites with different affinities. The fluorescence titration shows that Ce 3+ can bind to Ca 2+ binding sites.

关 键 词：rare earths CERIUM α  amylase activation 

分 类 号：Q55[生物学—生物化学]