APAAP桥联酶标技术快速诊断合胞病毒  

RAPID DIAGNOSIS OF RESPIRATORY SYNCYTIAL VIRUS INFECTION BY APAAP

作　　者：钱绩虎[1] 程纯[1] 王建明[1] 严靖[2] 

机构地区：[1]南通医学院微生物学教研室 [2]南通医学院附属医院儿科

出　　处：《南通医学院学报》1993年第2期99-101,共3页ACTA Academiae Medicinae Nantong

摘　　要：本文报告应用 APAAP 桥联酶标技术,对116例急性下呼吸道感染的患儿,进行合胞病毒抗原快速诊断研究,并与免疫荧光(IFA)技术对照.二者阳性符合率98.00%,阴性符合率95.38%,准确性为96.55%.进一步证明:APAAP 桥联酶标技术特异性好、敏感性高,用于呼吸道细胞检测,无内源性酶的干扰,且操作简便,无需荧光显微镜,利于基层推广应用,优于 IFA 技术,是较为理想的合胞病毒感染的快速诊断技术;若与 IFA 技术联用,可提高阳性检出率.Respiratory syncytial virus(RSV)is the most important cause of bronchiolitis and pneu- monia in infants and children.The APAAP was used to rapidly detect RSV antigen from 116 patients,compared with IFA method.The positive rate was conformed with 98.00%;The negative,95.38% and the accuracy,96.55%.Since alkaline phosphatase exists mainly in intes- tine,there are little enzyme in respiratory cells.The interference of endogenous enzyme can be avoided and results can be seen clearly under optical microscope.Thus the APAAP is a well-characterised,sensitive,and specific method for the rapid detection of RSV antigen. Then the combination APAAP with IFA can increase the detective rate of RSV antigen.

关 键 词：合胞病毒 诊断 桥联酶标技术 

分 类 号：R446.5[医药卫生—诊断学]