悬浮阵列及CCD凝结成像检测方法  

Suspension array and CCD freeze imaging detection method

作　　者：徐国雄[1] 黄震[1] 倪旭翔[1] 陆祖康[1] 

机构地区：[1]浙江大学光电系现代光学仪器国家重点实验室国家光学仪器工程技术研究中心

出　　处：《浙江大学学报（工学版）》2004年第11期1507-1510,1529,共5页Journal of Zhejiang University：Engineering Science

基　　金：国家自然科学基金资助项目(60378042).

摘　　要：研究了一种能对悬浮式生物芯片进行快速、准确、高灵敏度分析的测试技术,该技术采用电荷耦合器件(CCD)凝结成像技术检测悬浮式生物芯片中的分子反应信息.采用液流喷射技术建立平稳、均匀的二维流场.并行喷流悬浮式生物芯片溶液通过测试区域并采用CCD成像技术分析液流中的荧光信息,提高测试速度.研究用数字全息技术分析悬浮式生物芯片中微球探针的位置分布及尺寸分布,判别微球探针是否黏连,提高测试准确度.A rapid and accurate high-sensitivity system for suspension array detection was proposed. Molecules reaction information was obtained using charge coupled device (CCD) freeze imaging technology. Two-dimensional jet liquid of suspension biochip flowed through detective area and its Florence signal was analyzed by the system collaterally. Size and position distribution of micro beads in the suspension biochip were obtained in digital holography for testing whether the micro beads were glued to each other, so system accuracy was enhanced.

关 键 词：悬浮式阵列 二维流场 数字全息术 CCD凝结成像 

分 类 号：TH742.1[机械工程—光学工程]