兔出血症病毒(RHDV)McAb的研制及初步应用  

Study on Monoclonal Antibodies Against Rabbit Haemorrhagic Disease Virus (RHDV)

作　　者：贺争鸣[1] 巩薇[1] 梁红[1] 郎淑慧[1] 王秀清[1] 范文平[1] 卫礼[1] 吴惠英[1] 

机构地区：[1]中国药品生物制品检定所

出　　处：《中国实验动物学杂志》1998年第4期216-220,共5页Chinese Journal of Laboratory Animal Science

摘　　要：用酒石酸钾─甘油梯度离心纯化的RHDV抗原免疫BALB／C小鼠，取脾细胞与SP2／0融合．获得4株稳定分泌RHDVMcAb的杂交瘤细胞株（1F7，1H4，2A9，2D6）。经检测，所分泌的抗体亚类分别为IgG2a（1F，1H4，2D6）和IgG1（2A9）。应用双抗体夹心ELISA法检测70份标本，并与直接免疫荧光法和HA法比较，取得较好的实验结果。Four strains of hybridoma cells secreting speafic antibodies to RHDV were obtained by cellfusion technique. The RHDV used for immugon was purified by potassium Tartrafe-Glyceraldensity gradient Centrifugation. It was confirmed by western blot that the McAbs react with60KD, 55KD, 38KD and 20KD of RHDV protein. Double-McAb sandwich ELISA for the defection of RHDV antigen was established and analysis of sensitivity and specificity on test systemwas also performed in our laboratory.

关 键 词：分泌 杂交瘤细胞株 直接免疫荧光法 双抗体夹心ELISA法 脾细胞 HA 抗原免疫 兔出血症病毒 纯化 BALB/C小鼠 

分 类 号：Q939.4[生物学—微生物学] R392[医药卫生—免疫学]