1,6-二磷酸果糖对脑白质损害新生大鼠脑内β淀粉样前体蛋白表达的影响  

Effect of fructose-1,6-diphosphate on expression of β-amyloid precursor protein in white matter damage of neonatal rats.

作　　者：袁宝莉[1] 宋晓红[1] 李瑞林[1] 周戬平[1] 郭亚乐[1] 

机构地区：[1]西安交通大学第二医院儿科,陕西西安710004

出　　处：《中国儿童保健杂志》2004年第6期514-516,共3页Chinese Journal of Child Health Care

基　　金：陕西省自然科学研究计划项目 (2 0 0 1SM 77) ;西安交通大学第二医院 2 0 0 0年度院科研基金资助项目 (2 0 0 0KG 2 1 )

摘　　要：【目的】　探讨 1,6 二磷酸果糖 (fructose 1,6 diphosphate ,FDP)对脑白质损害 (whitematterdamage ,WMD)大鼠脑内 β淀粉样前体蛋白 (β amyloidprecursorprotein ,β APP)表达的影响。 　【方法】　 2日龄新生大鼠随机分为 :正常对照组、WMD组、FDP干预组及盐水干预组 ,后三组在建立新生大鼠脑白质损害模型后 ,应用相应干预方法进行干预。采用HE染色及免疫组织化学方法 ,观察损伤 2 4h后脑组织病理学结构和 β淀粉样前体蛋白表达的情况。　【结果】　实验各组HE染色未见明显差异 ,仅FDP干预组水肿稍减轻。WMD组及盐水干预组结扎侧β APP免疫反应较正常对照组明显增强 (P <0 .0 1) ,并且两组之间差异无显著性 (P >0 .0 5 )。FDP干预组结扎侧β APP免疫反应较WMD组盐水干预组明显减轻 (P <0 .0 5 )。 　【结论】　β APP是未成熟脑白质损伤的敏感指标。FDP可减少 β APP的表达水平 ,从而减轻缺血性脑损伤 ,对脑白质具有保护性作用。Objective To study the protective effect and value of fructose-1,6-diphosphate (FDP) on expression of β-amyloid precursor protein(β-APP) in white matter damage(WMD) of neonatal rats. The pups were randomly divided into normal control group, WMD group, FDP intervention group and normal saline intervention group.24 h after WMD models performed for the last 3 groups and received their own intervention method, HE and immunohistochemical technique was used to examine the structure and expression of β-APP in brain tissue of ligated side. There was no apparent abnormal in all groups at HE stains of all groups ,the edema of FDP intervention were less lighten.β-APP immunoreactivity of WMD group and normal saline intervention group enhanced ,FDP could delight the immunoreactivity of β-APP. [Conclusion] β-APP is a sensitive marker for axonal disruption in the white matter of the immature brain ,FDP could down regulation the expression of β-APP , from what lighten the ischemic brain injury , and protect the white matter of brain.

关 键 词：1 6-二磷酸果糖 Β淀粉样前体蛋白 脑白质损伤 

分 类 号：R722.1[医药卫生—儿科]