一种改进的水稻总DNA的快速提取方法  

An improved rapid method of plant total DNA extraction

作　　者：张凤娟[1] 张满良[1] 朱水芳 

机构地区：[1]西北农林科技大学植保学院,陕西杨凌712100 [2]中国检科院动植物检疫研究所

出　　处：《植物检疫》2004年第6期330-332,共3页Plant Quarantine

摘　　要：植物总DNA的提取质量是植物分子生物学操作成败的关键 ,一个优秀的总DNA提取方法往往可以达到事半功倍的效果。本文以水稻叶片为材料 ,提出了一种改进的植物叶片总DNA快速提取方法 ,该方法能在 35min之内提取到水稻总DNA。电泳结果表明此改进方法提取的总DNA产量与传统CTAB法相当 ,PCR扩增结果表明其质量可靠 。The isolation of plant chromosome DNA play a key role in biology. An improved plant chromosome DNA isolation method is developed, by which the plant chromosome DNA can be isolated successfully in 35 minutes. Electrophoresis results show that the quality of the DNA isolated with the improved method is equivalent to that of the DNA isolated with CTAB method, and the results of PCR indicate that the quality of the DNA isolated with the improved method can meet the requirements of plant molecular biology manipulation.

关 键 词：总DNA 水稻叶片 快速提取方法 CTAB法 优秀 产量 PCR扩增 植物分子生物学 植物叶片 电泳 

分 类 号：S511[农业科学—作物学] S563.1