白纹伊蚊经口感染、刺叮传播和经卵传递登革病毒的实验研究  

Experimental Study on Aedes albopictus by Orally Infected, Bitting and Transovarial Transmission with Dengue Virus

作　　者：林立辉[1] 方美玉[1] 陈翠华[1] 彭翼飞[1] 

机构地区：[1]广州军区军事医学研究所,广州510507

出　　处：《医学动物防制》2000年第5期225-228,共4页Journal of Medical Pest Control

基　　金：全军"九五"规划97L059重点项目;广东省卫生厅"九五;五个一科教兴医工程"重点项目

摘　　要：为评价白纹伊蚊在我国传播登革病毒(DEN)的媒介效能和了解自然界媒蚊体内病毒的保存机制,应用C_6/36细胞培养结合间接荧光抗体试验(IFA),研究经口感染DEN的白纹伊蚊,证明不仅对DEN有较高的感染率(32.1～32.8％),而且通过直接叮咬敏感乳鼠,也能传播DEN。对感染蚊的子_1和子_2代成蚊分别进行全蚊匀浆病毒分离和直接叮咬乳鼠法检测,也证明白纹伊蚊能经卵传递DEN,至少可传2代以上。说明白纹伊蚊对DEN能起较大的媒介效能。且在维持DEN的自然循环中,也起重要作用。Aedes albopictus by orally infected with dengue virus (DEN) Ⅰ , Ⅱ were detected by virus isolation in C6/36 cell culture combined with indirect fluorescent antibody(IFA). The infection rates of the whole mosquito were 32. 1 % and 32. 8% .and that of the salivary gland were 18. 4% and 18. 2% respectively. The study demonstrated Aedes albopictus could be infected by DEN. And DEN could be transmitting through directly biting infant mice and transovarial transmission. The results indicated that Aedes albopictus is important vector of dengne fever,its vector efficience was higher,and play an important role in maintenance of nature circulation of DEN.

关 键 词：白纹伊蚊 经口感染途径 刺叮传播 经卵传递 登革病毒 媒介效能 

分 类 号：R384.1[医药卫生—医学寄生虫学] R373.33[医药卫生—基础医学]