高产多糖钝顶螺旋藻新品系的选育及蛋白质SDS-PAGE鉴定  

SELECTION AND PROTEIN SDS-PAGE IDENTIFICATION OF A NEW HIGH-PRODUCING POLYSACCHARIDE STRAIN OF Spirulina platensis

作　　者：汪志平[1] 刘艳辉[1] 

机构地区：[1]浙江大学原子核农业科学研究所,浙江杭州310029

出　　处：《核农学报》2004年第5期349-352,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目 (3 0 0 0 0 0 10 )

摘　　要：研究了 1 0株钝顶螺旋藻对60 Coγ射线的辐射抗性及其与多糖含量的关系 ,表明各株系的半致死剂量 (LD50 )与多糖含量间的相关系数高达 0 9873。进而以多糖含量较高的钝顶螺旋藻Sp 0 8为出发品系、用 0 6%的EMS和 2 4kGy的60 Coγ射线处理Sp 0 8单细胞或原生质球 ,并用 7 0kGy左右的γ射线为筛选压力 ,得到了 4株多糖含量分别比Sp 0 8的高 32 8%、1 7 3%、2 3 4%和 42 3%的形态突变体 :Sp 0 8A、Sp 0 8B、Sp 0 8C和Sp 0 8D。蛋白质SDS PAGE分析确定 ,Sp 0 8A、Sp 0 8C和Sp 0 8D为在分子遗传水平发生变异的突变体。综合上述突变体的形态特征和生长速率等生产性状 ,确立Sp 0 8A为高产多糖的钝项螺旋藻新品系 。The relationship between anti radiation capacity and polysaccharide contents of ten Spirulina platensis strains were studied. The results showed that the correlation coefficient between 60 Co γ\|ray half lethal dose (LD 50 ) and polysaccharide contents of the 10 strains was 0 9873. After the single cells or spheroplasts of Sp 08 were treated by 0 6%EMS and 2 4 kGy 60 Co γ\|ray irradiation, four morphological mutant named Sp 08A,Sp 08B,Sp 08C and Sp 08D, which could endure about 7 0 kGy γ ray irradiation, were obtained, The polysaccharide contents of Sp 08A, Sp 08B, Sp 08C and Sp 08D, were 32 8%, 17 3%, 3 4% and 42 3% higher than that of their parent Sp 08, respectively. The results of protein SDS PAGE analysis showed that the heredity of Sp 08A, Sp 08C and Sp 08D were mutated. Therefore, Sp 08A was a perfect high producing polysaccharide strain with excellent characteristics of morphology and growth. Now, Sp 08A is applied in mass cultivation and industrialization.

关 键 词：新品系 高产 选育 突变体 株系 半致死剂量 生产性状 蛋白质S 多糖含量 ^60Coγ射线 

分 类 号：S968.4[农业科学—水产养殖]