小麦对黄花叶病的抗性鉴定及典型品种的遗传分析  

Identification of resistance to yellow mosaic disease of wheat and analysis for its inheritance of some varieties

作　　者：刘伟华[1] 何震天[2] 耿波[1] 侯明生[3] 张敏[4] 聂桓[1] 韩月澎[2] 韩成贵[1] 王锦荣[2] 于嘉林[1] 陈秀兰[2] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室 [2]江苏里下河地区农业科学研究所,扬州225207 [3]华中农业大学植保系,武汉430070 [4]四川农业大学农学院,雅安625014

出　　处：《植物病理学报》2004年第6期542-547,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金项目(30270874;39870489)资助

摘　　要：本研究针对来自江苏、河南、四川、湖北、日本和美国的37个小麦品种,在进行小麦抗黄花叶病的抗病性鉴定、调查的基础上,结合分子生物学检测(RT-PCR和ELISA),证实小麦品种扬辐9311对小麦黄花叶病毒(WYMV)表现免疫。将该品种与表现高感的小麦品种进行杂交和回交,通过对其后代抗感分离的调查与分析,确定了小麦品种扬辐9311对黄花叶病的抗性受一对显性基因控制,并为寻找小麦抗黄花叶病基因的分子标记提供理论依据和实验材料。同时,本文对小麦抗黄花叶病表现的影响因素及宁麦9号的抗病性进行了分析和讨论。Wheat yellow mosaic disease resistance was identified in the field among thirty-seven wheat varieties derived from China (Jiangsu, Henan, Sichuan and Hubei Province), Japan and United States. By RT-PCR and ELISA detections, it was proved that the wheat variety 'Yangfu 9311' displayed immunity to Wheat yellow mosaic virus(WYMV). The variety was crossed and backcrossed with those that were susceptible to the disease. By screening for the resistance among the segregating populations of F2 and BC1 the inheritance studies indicated that the resistance of 'Yangfu 9311' was controlled by a pair of dominant monogene. These results provided theoretical basis and experimental materials to find molecular markers of the resistance gene. In this paper, the resistant phenotype of Ningmai 9 and the influencing factors for resistance to Wheat yellow mosaic disease were also discussed.

关 键 词：花叶病 小麦品种 黄花 遗传分析 抗性鉴定 回交 抗病性鉴定 江苏 河南 四川 

分 类 号：S512.1[农业科学—作物学] S435